Dietary exogenous supplementation of nucleotides strengthens the disease resistance, antioxidant capacity and immunity in the gill of on-growing grass carp (Ctenopharyngodon idella) following a challenge with Flavobacterium columnare
Dietary exogenous supplementation of nucleotides strengthens the disease resistance, antioxidant capacity and immunity in the gill of on-growing grass carp (Ctenopharyngodon idella) following a challenge with Flavobacterium columnare
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DOI：
10.1016/j.aquaculture.2021.736729
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发表时间：
2021-04
期刊：
影响因子：
4.5
通讯作者：
Huaimao Tie;Lin Feng;Wei‐dan Jiang;Pei Wu;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Xiao‐qiu Zhou-Xia
中科院分区：
文献类型：
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作者：
Huaimao Tie;Lin Feng;Wei‐dan Jiang;Pei Wu;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Xiao‐qiu Zhou-Xia
低分子量核苷酸对鱼类和陆生动物具有重要的生物学作用，具有抗炎、抗菌、抗氧化、抗凋亡等多方面的生物学功能。本研究旨在探讨饲料中添加外源核苷酸对生长期草鱼（200.0 ± 1.0 g）鳃部抗病性、抗氧化能力、抗菌活性、炎性细胞因子及其相关信号传导机制的影响。在6种饲料中分别添加0、200.0、400.0、600.0、800.0和1000.0 mg/kg的核苷酸，连续饲喂60 d，然后用柱状黄杆菌攻毒3 d。结果表明，与0 mg/kg核苷酸组相比，600.0 mg/kg核苷酸组显著降低了F.抗氧化相关酶活性及相关基因表达显著增强（P< 0.05），部分归因于Nrf 2信号通路的调节（P< 0.05）。600.0 mg/kg核苷酸组显著提高了抗微生物物质（溶菌酶和IgM）的产生和抗微生物肽（如β-防御素）和Mucin-2的表达水平（P< 0.05），并改变了与NF-κB和TOR信号通路作用部分相关的主要炎症相关基因（如IL-1β和IL-8）的表达谱（P< 0.05）。而核苷酸组对TNF-α、IFN-γ、IL-12 p35和IL-4/13 B的表达无明显影响（P> 0.05）。饲料中添加外源核苷酸对提高草鱼鳃部抗病能力、抗氧化能力和免疫力具有潜在的益处，根据鳃部健康相关指标，饲料中外源核苷酸的适宜添加量为526.4-639.8 mg/kg。
Low-molecular-weight nucleotides are very important for fish and terrestrial animals, which have been verified to possess multidirectional biological functions including anti-inflammatory, anti-microbial, anti-oxidant, anti-apoptotic effects. This research was designed to fully explore the possible impacts of dietary exogenous nucleotides supplementation on gill disease resistance, antioxidant capacity, antibacterial activity, inflammatory cytokines and the associated signaling mechanisms in on-growing grass carp (Ctenopharyngodon idella) (200.0 ± 1.0 g). Fish were fed six diets containing graded levels of nucleotides at 0, 200.0, 400.0, 600.0, 800.0 and 1000.0 mg/kg in triplicate for 60 days, followed by a challenge withFlavobacteriumcolumnarefor 3 days. Our results showed that compared to 0 mg/kg nucleotides diet group, 600.0 mg/kg nucleotides diet group markedly decreased the gill rot morbidity of fish induced byF. columnare(P< 0.05), and significantly enhanced antioxidant related enzymes activities and corresponding gene expressions partly ascribed to the modulation of Nrf2 signaling (P< 0.05). Meanwhile, 600.0 mg/kg nucleotides diet group dramatically elevated antimicrobial substances (lysozyme and IgM) productions and antimicrobial peptides (such as β-defensin) and Mucin-2 expression levels (P< 0.05) as well as altered the expression profiles of main inflammatory-related genes (such as interleukin-1β and IL-8) partly correlated with the action of NF-κB and TOR signaling (P< 0.05). However, nucleotides diet groups did not alter the expressions of cytokines TNF-α, IFN-γ, IL-12p35 and IL-4/13B (P> 0.05). Dietary exogenous nucleotides supplementation has potential benefits in strengthening gill disease resistance, antioxidant capacity and immunity in grass carp, the optimal levels of dietary exogenous nucleotides supplementation are recommended to be 526.4–639.8 mg/kg based on gill health-related indices.