Modulation of the Silica Sol-Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c
Modulation of the Silica Sol-Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c
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DOI：
10.1021/la5023517
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发表时间：
2014-09-02
期刊：
影响因子：
3.9
通讯作者：
Montilla, Francisco
中科院分区：
文献类型：
--
作者：
Gamero-Quijano, Alonso;Huerta, Francisco;Montilla, Francisco
研究了不同溶胶-凝胶二氧化硅网络包裹的氧化铟锡电极（ITO）和细胞色素c之间的直接电子转移。以含溶解细胞色素c的磷酸盐缓冲液和无醇溶胶-凝胶法制备的硅溶胶混合，采用两步包封法制备Cyt c@silica修饰电极。用循环伏安法、紫外-可见光谱法和原位紫外-可见光谱电化学对这些修饰电极进行了表征。包裹蛋白的电化学响应受二氧化硅孔末端基团的影响。Cyt c在传统的二氧化硅（羟基端）或苯基端二氧化硅中不存在电化学反应。当蛋白质被包裹在甲基修饰的二氧化硅网络中时，直接电子转移到被包裹的细胞色素c和ITO电极上。
The direct electron transfer between indium-tin oxide electrodes (ITO) and cytochrome c encapsulated in different sol-gel silica networks was studied. Cyt c@silica modified electrodes were synthesized by a two-step encapsulation method mixing a phosphate buffer solution with dissolved cytochrome c and a silica sol prepared by the alcohol-free sol-gel route. These modified electrodes were characterized by cyclic voltammetry, UV-vis spectroscopy, and in situ UV-vis spectroelectrochemistry. The electrochemical response of encapsulated protein is influenced by the terminal groups of the silica pores. Cyt c does not present electrochemical response in conventional silica (hydroxyl terminated) or phenyl terminated silica. Direct electron transfer to encapsulated cytochrome c and ITO electrodes only takes place when the protein is encapsulated in methyl modified silica networks.