Preliminary data on cellulase encoding genes in the xylophagous beetle, Hylotrupes bajulus (Linnaeus)
Preliminary data on cellulase encoding genes in the xylophagous beetle, Hylotrupes bajulus (Linnaeus)
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DOI：
10.1016/j.ibiod.2013.09.009
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发表时间：
2014-01-01
影响因子：
4.8
通讯作者：
Chiappini, Elisabetta
中科院分区：
文献类型：
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作者：
Busconi, Matteo;Berzolla, Alessia;Chiappini, Elisabetta
bajulus Hylotrupes (Linnaeus)（鞘翅目，天牛科），一种以软木为食的蛀木甲虫，以木材淀粉和纤维素为食物。纤维以不同的百分比被消化，这取决于淀粉含量。据推测，共生细菌以及内源性纤维素酶的存在也起到了一定的作用。我们的研究目的是验证内源性内切葡聚糖酶基因的存在，在H. bajulus幼虫。通过在线检索，我们找到了GHF5类内切葡聚糖酶的3个序列和GHF45类内切葡聚糖酶的4个序列，并对其进行了比对，以便在最保守的区域开发引物，并尝试在H. bajulus中扩增基因。测试了7条GHF5内切葡聚糖酶和5条GHF45内切葡聚糖酶的退化引物。通过使用两种不同的引物组合，我们得到了两个片段：它们都与昆虫的GHF45家族的内切葡聚糖酶具有显著的同源性，一个也与微生物的纤维素酶具有显著的同源性。第一个片段无疑是内源性的，而第二个片段则不排除是微生物的。未获得GHF5类内切葡聚糖酶的结果。(C) 2013 Elsevier Ltd.版权所有。
Hylotrupes bajulus (Linnaeus) (Coleoptera, Cerambycidae), a wood-boring beetle attacking softwood, utilises both wood starch and cellulose as food. The fibre is digested in variable percentages, depending on the starch content. A role for symbiotic bacteria was presumed as well as the presence of endogenous cellulases. The aim of our investigation was to verify the presence of endogenous endoglucanase genes in H. bajulus larvae. After performing an online search, three sequences for the endoglucanases of the class GHF5 and four for the endoglucanases of the class GHF45 were recovered and aligned in order to develop some primers on the most conserved regions and try the amplification of the genes in H. bajulus. Seven degenerated primers for GHF5 endoglucanase and five for GHF45 endoglucanase were tested. By using two different primer combinations we obtained two fragments: both of them showed homology with significant identity with insect endoglucanases of the GHF45 family available online, and one also with microbial cellulases. The first fragment is undoubtedly of endogenous origin while we cannot exclude that the second one is of microbial origin. No results were obtained for the endoglucanase of the class GHF5. (C) 2013 Elsevier Ltd. All rights reserved.