Optimization and characterization of a flow cell for heat-transfer-based biosensing
Optimization and characterization of a flow cell for heat-transfer-based biosensing
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DOI：
10.1002/pssa.201600758
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发表时间：
2017-09-01
影响因子：
2
通讯作者：
Eersels, Kasper
中科院分区：
文献类型：
--
作者：
Stilman, Wouter;Jooken, Stijn;Eersels, Kasper
在这篇文章中，我们报告了一种流动电池的发展优化的传热方法，一种多功能的生物传感技术。流动电池的设计确保热流集中，通过电池周围环境的热损失最小。这使得测量信号更加稳定，并且提高了测量技术的灵敏度。该传感器的特点是在空气、水和磷酸盐缓冲盐水（PBS）溶液中进行背景测量。利用COMSOL模拟了通过该装置的热流，以便深入了解对流对热流的贡献，并对电池未来可能的改进提出建议。此外，定义了计算热阻的两步算法，使用户能够从实验数据中准确地获得导热系数。最后，在一个类似的实验中，评估了流动池对细菌（大肠杆菌）检测的潜力，并将其与原始HTM设置的结果进行了比较。该实验表明，通过改变测量单元的几何形状，我们能够将检测限（LoD）提高到2.10x10(4)个菌落形成单位（CFU） mL（-1）。用于热生物检测实验的传感器设置有定向热流。(C) 2016 WILEY-VCH Verlag GmbH & Co., KGaA, Weinheim
In this article, we report on the development of a flow cell optimized for the heat-transfer method, a versatile biosensing technique. The design of the flow cell ensures that the heat flow is focused with minimal heat loss through the surroundings of the cell. This results in a more stable measuring signal and an improved sensitivity of the measuring technique. The sensor was characterized by performing background measurements in air, water, and phosphate buffered saline (PBS) solution. Heat flow through the setup was simulated using COMSOL in order to provide insight in the contribution of convection to the heat flow and recommendations for possible future improvements to the cell. Additionally, a two-step algorithm for calculating thermal resistance was defined, allowing the user to accurately derive thermal conductivity from experimental data. Finally, the potential of the flow cell for bacteria (Escherichia coli) detection was assessed and compared with the results obtained in the original HTM setup in a similar experiment. This experiment demonstrates that we were able to improve the limit-of-detection (LoD) to 2.10x10(4) colony forming units (CFU) mL(-1) by changing the geometry of the measuring cell.[GRAPHICS].Sensor setup for thermal biodetection experiments a directed heat flow. (C) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim