REPEATED ADMINISTRATION OF PROSTAGLANDIN-F(2-ALPHA) DURING THE EARLY LUTEAL-PHASE CAUSES PREMATURE LUTEOLYSIS IN THE PIG
REPEATED ADMINISTRATION OF PROSTAGLANDIN-F(2-ALPHA) DURING THE EARLY LUTEAL-PHASE CAUSES PREMATURE LUTEOLYSIS IN THE PIG
复制标题
DOI：
10.1095/biolreprod49.1.181
复制
发表时间：
1993-07-01
影响因子：
3.6
通讯作者：
GADSBY, JE
中科院分区：
文献类型：
--
作者：
ESTILL, CT;BRITT, JH;GADSBY, JE
之前的研究者认为猪黄体在发情周期第12天前对前列腺素（PG）F2 α的溶黄体作用不敏感。本研究旨在确定多次注射PGR 2 α是否会导致血清孕酮和黄体溶解持续降低，从而导致发情周期和发情间期显著缩短。在发情周期第5-10天，后备母猪（n = 4）接受12.5 mg PGF 2 α（地诺前列素氨丁三醇）i.m.注射。每12小时，或媒介物（PBS; n = 4）。PGF 2 α给药后备母猪的平均间期缩短（p < 0.001）至13.3 +/- 0.5天，而对照后备母猪为19.8 +/- 0.6天。与对照动物第17.5天相比，PGF 2 α给药后备母猪第10.5天的血清孕酮下降至1 ng/ml以下。 PGF 2 α给药后备母猪的雌二醇-17 β（E2）血清浓度在周期内（第12.5天）比对照后备母猪（第19.5天）更早达到最大水平。E2和LH峰值浓度与围动情期一致，表明PGF 2 α诱导的发情伴随着正常的卵泡发育和排卵。这些结果表明，如果从第5天至第10天重复注射，则猪在第12天前对PGF 2 α的溶黄体作用敏感。
Previous investigators considered pig corpora lutea refractory to the luteolytic effects of prostaglandin (PG) F2alpha before Day 12 of the estrous cycle. This study was designed to determine whether multiple injections of PGR2alpha would result in a sustained reduction of serum progesterone and luteolysis, leading to significant shortening of the estrous cycle and interestrous interval. On Days 5-10 of an estrous cycle, gilts (n = 4) received injections of 12.5 mg PGF2alpha (dinoprost tromethamine) i.m. every 12 h, or vehicle (PBS; n = 4) according to the same schedule. Mean interestrous interval in PGF2alpha-treated gilts was reduced (p < 0.001) to 13.3 +/- 0.5 days compared with 19.8 +/- 0.6 days for control gilts. Serum progesterone declined below 1 ng/ml by Day 10.5 in PGF2alpha-treated gilts compared to Day 17.5 in control animals. Serum concentrations of estradiol-17beta (E2) reached maximal levels in PGF2alpha-treated gilts earlier (Day 12.5) in the cycle than in control gilts (Day 19.5). Peak E2 and LH concentrations coincided with the periestrous period, suggesting that PGF2alpha-induced estrus is accompanied by normal follicular development and ovulation. These results demonstrate that the pig is susceptible to the luteolytic effects of PGF2alpha before Day 12 if repeated injections are given from Day 5 through Day 10.