Blood meal origins and insecticide susceptibility of Anopheles arabiensis from Chano in South-West Ethiopia
Blood meal origins and insecticide susceptibility of Anopheles arabiensis from Chano in South-West Ethiopia
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DOI：
10.1186/1756-3305-6-44
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发表时间：
2013-02-22
影响因子：
3.2
通讯作者：
Lindtjorn, Bernt
中科院分区：
文献类型：
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作者：
Massebo, Fekadu;Balkew, Meshesha;Lindtjorn, Bernt
背景：阿拉伯按蚊是埃塞俄比亚的主要疟疾病媒，具有嗜人和嗜动物两种行为。杀虫剂的抗药性正在增加，因此需要采用其他的病媒控制方法。本研究的目的是确定血粉来源和对杀虫剂的敏感性。方法：采用直接酶联免疫吸附试验（ELISA），对按蚊的血餐来源进行分析，血餐来源采用美国疾病控制和预防中心（CDC）的捕蚊灯和除虫菊喷雾捕蚊器（PSC）从人类住所采集，手持口吸器从室外防空洞采集。的易感性。结果：测定了2967只新鲜饲养的阿拉伯按蚊对拟除虫菊酯类杀虫剂（氯氰菊酯、氟氯氰菊酯、溴氰菊酯和氟氯氰菊酯）和DDT的血食来源。一个. arabiensis是主要物种（75%），主要以牛为食。两种新饲养的安。阿拉伯虎和以人血为食的虎也遵循类似的季节模式。A.人血总指数（HBI）包括混合血粉在内的阿拉伯牛血液指数（BBI）为44%，牛血液指数（BBI）为69%。的HBI。来自CDC灯诱收集物的BBI为75%，并且这高于PSC（38%）和室外坑掩蔽物收集物（13%）的BBI，而BBI对于PSC为65%，对于室外坑掩蔽物为68%，并且对于CDC灯诱收集物为72%。更多的新鲜喂养和人类血液喂养的安。从阿巴亚湖（主要繁殖地）海岸附近的房屋中取样。阿拉伯按蚊对拟除虫菊酯类杀虫剂产生抗药性，对三氟氯氰菊酯的致死率为56%，对氟氯氰菊酯和高效氯氰菊酯的致死率为50%，对溴氰菊酯的致死率为47%，对DDT的致死率为10%。该物种更倾向于以牛为食，因此有理由对牛使用杀虫剂来控制它。阿拉伯按蚊已经对现有的拟除虫菊酯类杀虫剂产生了抗药性，需要替代杀虫剂来控制疟疾病媒。
Background: Anopheles arabiensis, the main malaria vector in Ethiopia, shows both anthropophilic and zoophilic behaviours. Insecticide resistance is increasing, and alternative methods of vector control are needed. The objectives of this study were to determine the blood meal origins and the susceptibility to insecticides of An. arabiensis from Chano village near Arba Minch in South-West Ethiopia.Methods: Blood meal sources of anopheline mosquitoes collected using Centers for Disease Control and Prevention (CDC) light traps and pyrethrum spray catches (PSC) from human dwellings, and hand-held mouth aspirators from outdoor pit shelters were analysed using a direct enzyme-linked-immunosorbent assay (ELISA). The susceptibility of An. arabiensis to pyrethroid insecticides (alphacypermethrin, lambdacyhalothrin, deltamethrin, and cyfluthrin) and DDT was assessed using females reared from larval and pupal collections from natural breeding sites.Results: The blood meal origins of 2967 freshly fed Anopheles mosquitoes were determined. An. arabiensis was the predominant species (75%), and it fed mainly on cattle. The densities of both freshly fed An. arabiensis and those fed on human blood followed similar seasonal patterns. The overall human blood index (HBI) of An. arabiensis, including mixed blood meals, was 44% and the bovine blood index (BBI) was 69%. The HBI of An. arabiensis from CDC light trap collections was 75% and this was higher than those for PSC (38%) and outdoor pit shelter collections (13%), while the BBI was 65% for PSC, 68% for outdoor pit shelters and 72% for CDC light traps. More freshly fed and human blood-fed An. arabiensis were sampled from houses close to the shore of Lake Abaya (the major breeding site).A high proportion of An. arabiensis was resistant to the pyrethroid insecticides, with a mortality rate of 56% for lambdacyhalothrin, 50% for cyfluthrin and alphacypermethrin, 47% for deltamethrin, and 10% for DDT.Conclusion: Anopheles arabiensis is the predominant species of anopheline mosquito in this region, and cattle are the main source of its blood meals. The greater tendency of this species to feed on cattle justifies the application of insecticides on cattle to control it. However, An. arabiensis has already developed resistance to the available pyrethroid insecticides, and alternative insecticides are needed for malaria vector control.