ISOLATION AND FUNCTIONAL-ANALYSIS OF A HUMAN 70,000-DALTON HEAT-SHOCK PROTEIN GENE SEGMENT
ISOLATION AND FUNCTIONAL-ANALYSIS OF A HUMAN 70,000-DALTON HEAT-SHOCK PROTEIN GENE SEGMENT
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DOI：
10.1073/pnas.82.15.4949
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
RUNGGER, D
中科院分区：
文献类型：
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作者：
VOELLMY, R;AHMED, A;RUNGGER, D
人70 kDa [千道尔顿]热休克蛋白（hsp 70）基因片段的分离。该片段包含3.15个5“非转录序列的双链酶对（kbp）、119 bp的RNA前导序列和741 bp的蛋白质编码区。人蛋白质序列显示出与来自其他物种的HSP 70序列高度同源性。在非洲爪蟾卵母细胞和哺乳动物细胞中的表达实验表明，仅包括105 bp的5“非转录序列的区域包含有效热控制表达人类基因所需的所有元件。两个相邻的相同的序列元件，这是部分同源的果蝇热休克的共识序列，位于57-76 bp上游的帽位点。有趣的是，加帽位点本身两侧是反向重复序列。
A human 70-kDa [kilodalton] heat shock protein (hsp70) gene segment was isolated. The segment contains 3.15 kilobase pairs (kbp) of 5'' nontranscribed sequence, an RNA leader of 119 bp [base pairs], and a protein-coding region of 741 bp. The human protein sequence shows a high degree of homology to hsp 70 sequences from other species. Expression experiments in Xenopus oocytes and mammalian cells indicate that a region that includes only 105 bp of 5'' nontranscribed sequence contains all elements required for the efficient heat-controlled expression of the human gene. Two adjacent identical sequence elements, which are partly homologous to the Drosophila heat shock consensus sequence, are located 57-76 bp upstream from the capping site. Interestingly, the capping site itself is flanked by inverted repeat sequences.