Overcoming Cross-incompatibility between Nicotiana tabacum L. and N.rustica L. by Test-tube Pollination and Ovule Culture
Overcoming Cross-incompatibility between Nicotiana tabacum L. and N.rustica L. by Test-tube Pollination and Ovule Culture
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通过试管授粉和胚珠培养克服烟草和黄花烟草之间的交叉不亲和性
DOI：
10.1270/jsbbs1951.35.429
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
T. Nakajima
中科院分区：
文献类型：
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作者：
W. Marubashi;T. Nakajima
由于烟叶（=）与野蔷薇（N.rustica）存在杂交不亲和性，无法通过常规杂交获得种间杂种。采用试管授粉和胚珠培养相结合的方法克服了杂交不亲和组合。采用粉粒法对烟粉虱全胎盘和完整胚珠进行离体授粉。从胎盘中取出成熟的胚珠，转移到胚珠培养中。通过两种技术的组合，获得了12个杂交植株。杂交植株的形态形态介于亲本之间，有48条染色体，是亲本单倍体染色体的加和。它们不能产生可育的花粉，完全自我不育。经秋水仙碱处理，获得了可育的双染色体杂种。综上所述，试管授粉和胚珠培养相结合的方法可以有效地克服远亲种间的杂交不亲和性。
Interspecific hybrids between Nicotiana tabacum (=) and N.rustica can not be obtained by conventional crossing because of cross-incompatibilit.y. Two techniques, test-tube pollination and ovule culture, were combined to overcome cross-incompatible combination. Whole placentae with intact ovules of N.tabacum were pollinated in vitro by dusting pollen grains of N.rustica. Developed ovules were excised from the placentae and transferred to ovule culture. Finally, 12 hybrid plants were obtained by the combination of these two techniques. The hybrid plants were intermediate between their parents in their morphological appearance and had 48 chromosomes which were the additive sum of the haploid chromosomes of the parents. They failed to produce fertile pollen and were completely self-sterile. A fertile chromosome-doubled hybrid was obtained by colchicine treatment. From the results, it is considered that the combination of two techniques, test-tube pollination and ovule culture, is useful for overcoming cross-incompatibility between distantly related species.