Distribution of short neuropeptide F and its receptor in neuronal circuits related to feeding in larval Drosophila
Distribution of short neuropeptide F and its receptor in neuronal circuits related to feeding in larval Drosophila
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DOI：
10.1007/s00441-013-1660-4
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发表时间：
2013-09-01
影响因子：
3.6
通讯作者：
Nassel, Dick R.
中科院分区：
文献类型：
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作者：
Carlsson, Mikael A.;Enell, Lina E.;Nassel, Dick R.
来自snpf基因的四种形式的短神经肽F （sNPF1-4）已经在果蝇中被鉴定出来，并且已知它们作用于单个g蛋白偶联受体（sNPFR）。在果蝇中，sNPFs的一些功能已经被提出，包括调节幼虫的摄食和生长，控制胰岛素信号和调节成年果蝇的神经回路。此外，sNPF已被证明在嗅觉系统中作为一种营养状态依赖的神经调节剂。sNPF在幼虫神经系统中的作用尚不为人所知。为了分析sNPF在幼虫中的作用位点，我们绘制了sNPF-和sNPF-表达神经元的分布图。特别是，我们研究了与化学感觉输入相关的电路和参与摄食调节的系统，包括神经分泌细胞系统和下脑神经节。我们采用免疫细胞化学、增强子陷阱和启动子Gal4系相结合的方法来驱动绿色荧光蛋白。我们发现受体的分布与其配体之间有很好的匹配。然而，幼虫和成虫系统之间存在一些差异。因此，在幼虫的嗅觉（或其他感觉）系统中都没有发现sNPFR及其受体，并且产生胰岛素样肽的细胞没有共同表达sNPFR，这与成虫的结果相反。此外，sNPF仅在成年果蝇的Hugin细胞（二级味觉神经元）亚群中表达。我们提出sNPF信号在发育阶段之间的差异可以通过它们摄食行为的差异来解释。
Four forms of short neuropeptide F (sNPF1-4), derived from the gene snpf, have been identified in Drosophila and are known to act on a single G-protein-coupled receptor (sNPFR). Several functions have been suggested for sNPFs in Drosophila, including the regulation of feeding and growth in larvae, the control of insulin signalling and the modulation of neuronal circuits in adult flies. Furthermore, sNPF has been shown to act as a nutritional state-dependent neuromodulator in the olfactory system. The role of sNPF in the larval nervous system is less well known. To analyse sites of action of sNPF in the larva, we mapped the distribution of sNPF- and sNPFR-expressing neurons. In particular, we studied circuits associated with chemosensory inputs and systems involved in the regulation of feeding, including neurosecretory cell systems and the hypocerebral ganglion. We employed a combination of immunocytochemistry and enhancer trap and promoter Gal4 lines to drive green fluorescent protein. We found a good match between the distribution of the receptor and its ligand. However, several differences between the larval and adult systems were observed. Thus, neither sNPF nor its receptor was found in the olfactory (or other sensory) systems in the larva and cells producing insulin-like peptides did not co-express sNPFR, as opposed to results from adults. Moreover, sNPF was expressed in a subpopulation of Hugin cells (second-order gustatory neurons) only in adult flies. We propose that the differences in sNPF signalling between the developmental stages is explained by differences in their feeding behaviour.