Silver deposited polystyrene (PS) microspheres for surface-enhanced Raman spectroscopic-encoding and rapid label-free detection of melamine in milk powder
Silver deposited polystyrene (PS) microspheres for surface-enhanced Raman spectroscopic-encoding and rapid label-free detection of melamine in milk powder
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DOI：
10.1016/j.talanta.2013.03.075
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发表时间：
2013-09-15
期刊：
影响因子：
6.1
通讯作者：
Huan, Shuangyan
中科院分区：
文献类型：
--
作者：
Zhao, Yanhua;Luo, Weiqi;Huan, Shuangyan
通过精确控制沉积时间，制备了表面银纳米粒子包覆的氨基修饰聚苯乙烯微球（PS-NH 2/Ag NPs），该微球具有极高的表面增强拉曼散射（Sers）活性和均匀的表面形貌。在单个微球下方形成的纳米射流可用于对吸附在光滑的金或玻璃表面上的物质进行无损分析。与玻璃表面相比，PS-NH 2/Ag NPs与金表面之间的Sers增强了10倍，这是由于更有效的表面等离子体共振耦合。这些微球Sers基底可以检测低至10（-9）M的2-巯基吡啶。四种不同的硫醇化合物已成功地用作标签，制备Sers编码的PS-NH 2/Ag NPs微球。此外，这些Sers基底的潜在应用，快速检测奶粉中的三聚氰胺进行了探索。结果表明，Sers强度与三聚氰胺浓度的对数呈线性关系，检测限为1.9 × 10（-9）mol/L。由于PS-NH 2/Ag纳米粒子具有样品前处理简单、蛋白质背景干扰小、检测时间短、成本低等优点，因此有望成为食品安全领域的一种潜在检测工具。(C)2013爱思唯尔有限公司版权所有。
Silver nanoparticles coated amino modified polystyrene microspheres (PS-NH2/Ag NPs) with extremely high surface enhanced Raman scattering (SERS) activity and uniform surface morphology were created by precise controlling of deposition time. Nanojets that were formed underneath the individual microspheres could be used for nondestructive analysis of species adsorbed on the smooth gold or glass surface. A 10 fold enhancement of SERS was observed between PS-NH2/Ag NPs and gold surface compared to glass surface due to more effective coupling of surface plasmonic resonance. These microsphere SERS substrates could detect 2-Mercaptopyridine down to 10(-9)M. Four different thiol compounds have been successfully utilized as tags to prepare SERS encoded PS-NH2/Ag NPs microspheres. Furthermore, the potential application of these SERS substrate for rapid detection of melamine in milk powder was explored. A linear relationship was observed between SERS intensity and logarithm of melamine concentrations with the limit of detection (LOD) of 1.9 x 10(-9) mol/L The promising advantages of easy sample pretreatment, low protein background interference, short detection time and low cost makes the PS-NH2/Ag NPs substrate a potential detection tool in the field of food safety. (C) 2013 Elsevier B.V. All rights reserved.