Growth response to ionic and osmotic stress of NaCl in salt-tolerant and salt-sensitive maize.
Growth response to ionic and osmotic stress of NaCl in salt-tolerant and salt-sensitive maize.
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DOI：
10.1111/j.1744-7909.2010.00947.x
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发表时间：
2010-05
影响因子：
11.4
通讯作者：
K. Zhao;Jie Song;H. Fan;San Zhou;Meng Zhao
中科院分区：
文献类型：
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作者：
K. Zhao;Jie Song;H. Fan;San Zhou;Meng Zhao
用100 mM NaCl处理耐盐玉米（STM）和盐敏感玉米（SSM）1、3和6 d，用X射线微区分析法测定了叶片细胞不同细胞器中Na+和Cl（-）（cps）含量。结果表明，Na+和Cl（-）同时进入细胞质、液泡、叶绿体和质外体。当在大气压（-P）和与NaCl（+P）的渗透压相等的压力下在100 mM NaCl中处理STM和SSM时，STM（+P）和SSM（+P）植物的干重大于STM（-P）和SSM（-P）植物的干重，表明盐对植物生长的抑制作用至少部分是由于NaCl的渗透作用。当STM和SSM用NaCl和等渗聚乙二醇处理时，给予等渗聚乙二醇处理的植物的干重对于两个玉米品系都低于NaCl处理的植物。我们的数据表明，在NaCl胁迫下，STM和SSM幼苗同时遭受渗透和离子胁迫。
Salt-tolerant maize (STM) and salt-sensitive maize (SSM) were treated with 100 mM NaCl for 1, 3 and 6 d and the contents of Na+ and Cl(-) (cps) of different organelles of leaf cells determined by X-ray microanalysis. The results showed that Na+ and Cl(-) entered the cytoplasm, vacuole, chloroplast and apoplast simultaneously. When STM and SSM were treated in 100 mM NaCl at atmospheric pressure (-P) and with pressure equivalent to the osmotic pressure of the NaCl (+P), the dry weights of STM (+P) and SSM (+P) plants were greater than that of STM (-P) and SSM (-P) plants, showing that the inhibitory effect of salt on plant growth was at least partially due to the osmotic effect of the NaCl. When STM and SSM were treated with NaCl and iso-osmotic polyethlene glycol, the dry weights of plants given the iso-osmotic polyethlene glycol treatment were lower for both maize lines than that of the NaCl-treated plants. Our data show that under NaCl stress, both STM and SSM seedlings simultaneously suffered from osmotic and ion stresses.