Detection of high-risk HPV DNA in semen and its association with the quality of semen
Detection of high-risk HPV DNA in semen and its association with the quality of semen
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DOI：
10.1258/0956462042395122
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发表时间：
2004-11-01
影响因子：
1.4
通讯作者：
Syrjänen, SM
中科院分区：
文献类型：
--
作者：
Rintala, MAM;Grénman, SE;Syrjänen, SM
评估精液中高危型人乳头状瘤病毒（HPV）DNA对精液质量的影响。收集了参与正在进行的芬兰HPV家族研究的65名男性的精液样本。精液分析是根据北欧男科协会的指南进行的。用巢式聚合酶链反应（PCR）检测高危型HPV DNA，Southern杂交证实高危型HPV DNA阳性，精液中高危型HPV DNA阳性率为15.4%（10/65）。精液中高危型HPV DNA不影响精液量、精子密度、精子活动率和活力。然而，HPV DNA阳性的精液pH值低于阴性样本（7.4 vs 7.5）。无论是少精症还是弱精症都与精液HPV DNA无关。总之，在15%的男性中检测到精液高危型HPV DNA。它不影响精液分析，除了精液pH值的边缘意义。捐精者尚未接受HPV感染检测，精子洗涤似乎不能消除HPV传播的风险，精液中HPV的后果目前尚不清楚。
The effects of seminal high-risk human papillornavirus (HPV) DNA were assessed on the quality of semen. Semen samples of 65 men participating in the ongoing Finnish HPV Family Study were collected. Semen analyses were done by the guidelines of the Nordic Association for Andrology. HPV DNA was detected by nested polymerase chain reaction and confirmed by Southern blot hybridization for high-risk types.Altogether, 10/65 men (15.4%) had high-risk HPV DNA positive semen sample. Seminal high-risk HPV DNA did not affect semen volume, sperm concentration, motility and vitality of spermatozoa. However, semen pH was borderline lower in HPV DNA positive than negative samples (7.4 vs 7.5). Neither oligo- nor asthenozoospermia was associated with seminal HPV DNA.In conclusion, seminal high-risk HPV DNA was detected in 15% of men. It did not affect the semen analysis, except semen pH by borderline significance. Sperm donors have not been tested for HPV infections, sperm washing does not seem to eliminate the risk of HPV transmission and the consequences of HPV in the semen are at present unknown.