GLUT4 is reduced in slow muscle fibers of type 2 diabetic patients - Is insulin resistance in type 2 diabetes a slow, type 1 fiber disease?
GLUT4 is reduced in slow muscle fibers of type 2 diabetic patients - Is insulin resistance in type 2 diabetes a slow, type 1 fiber disease?
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DOI：
10.2337/diabetes.50.6.1324
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发表时间：
2001-06-01
期刊：
影响因子：
7.7
通讯作者：
Handberg, A
中科院分区：
文献类型：
--
作者：
Gaster, M;Staehr, P;Handberg, A
为了进一步了解肌肉胰岛素抵抗的机制，研究了肥胖和2型糖尿病对慢和快骨骼肌纤维中GLUT4免疫反应性的影响。通过一种新开发的，非常敏感的方法，使用免疫组织化学结合形态测定，GLUT4密度被发现是显着较高的慢与快纤维活检标本从瘦和肥胖的主题。相反，在2型糖尿病患者中，慢纤维中的GLUT4密度显著低于快纤维。与体重匹配的肥胖受试者相比，糖尿病患者慢纤维中的GLUT4密度降低了9%，与瘦对照组相比降低了18%。与对照组相比，肥胖受试者的慢纤维分数降低至86%，糖尿病受试者降低至75%。与对照组相比，肥胖受试者中慢纤维的GLUT4贡献估计值降低至77%，2型糖尿病患者中降低至61%。我们建议。慢收缩纤维部分的减少与慢纤维中GLUT4表达的减少相结合，可减少2型糖尿病中胰岛素敏感性GLUT4库，从而导致骨骼肌胰岛素抵抗。
To gain further insight into the mechanisms underlying muscle insulin resistance, the influence of obesity and type 2 diabetes on GLUT4 immunoreactivity in slow and fast skeletal muscle fibers was studied. Through a newly developed, very sensitive method using immunohistochemistry combined with morphometry, GLUT4 density was found to be significantly higher in slow compared with fast fibers in biopsy specimens from lean and obese subjects. In contrast, in type 2 diabetic subjects, GLUT4 density was significantly lower in slow compared with fast fibers. GLUT4 density in slow fibers from diabetic patients was reduced by 9% compared with the weight-matched obese subjects and by 18% compared with the lean control group. The slow-fiber fraction was reduced to 86% in the obese subjects and to 75% in the diabetic subjects compared with the control group. Estimated GLUT4 contribution from slow fibers was reduced to 77% in the obese subjects and to 61% in type 2 diabetic patients compared with the control subjects. We propose that. a reduction in the fraction of slow-twitch fibers, combined with a reduction in GLUT4 expression in slow fibers, may reduce the insulin-sensitive GLUT4 pool in type 2 diabetes and thus contribute to skeletal muscle insulin resistance.