Molecular analysis of PML‐RARa fusion mRNA detected by reverse transcription‐polymerase chain reaction assay in long‐term disease‐free acute promyelocytic leukaemia patients
Molecular analysis of PML‐RARa fusion mRNA detected by reverse transcription‐polymerase chain reaction assay in long‐term disease‐free acute promyelocytic leukaemia patients
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DOI：
10.1111/j.1365-2141.1995.tb05228.x
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发表时间：
1995-08
影响因子：
6.5
通讯作者：
G. Martinelli;C. Remiddi;G. Visani;P. Farabegoli;N. Testoni;A. Zaccaria;S. Manfroi;A. Cenacchi;D. Russo;G. Bandini;M. Amabile;A. Vivo;S. Tura
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文献类型：
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作者：
G. Martinelli;C. Remiddi;G. Visani;P. Farabegoli;N. Testoni;A. Zaccaria;S. Manfroi;A. Cenacchi;D. Russo;G. Bandini;M. Amabile;A. Vivo;S. Tura
总结。通过使用PML/RARa的RT - PCR，我们评估了10例APL患者在诱导化疗和巩固后长期无疾病状态下的骨髓抽吸情况（中位54个月；从完全缓解到33 - 101个月）。在分子评估时，所有患者均处于临床和细胞遗传学缓解期（CR后32 - 96个月）。除1例患者外，所有患者在分子水平上PML - RARa转录物的表达均为RT - PCR阴性，这证实了大多数APL长期生存患者的特征是肿瘤克隆的根除。
Summary. By use of RT‐PCR of PML/RARa, we evaluated bone marrow aspirates in 10 patients with APL in long‐term disease‐free status after induction chemotherapy and consolidation (median 54 months; range 33‐101 months from complete remission). All patients were in clinical and cytogenetic remission at the time of molecular evaluation (range 32‐96 months from CR). All patients but one were found to be RT‐PCR negative at the molecular level for the expression of PML‐RARa transcript, confirming that the majority of the patients with long‐term survival of APL are characterized by the eradication of the neoplastic clone.