CONTINUED EXPRESSION OF HPV-16 E7 PROTEIN IS REQUIRED FOR MAINTENANCE OF THE TRANSFORMED PHENOTYPE OF CELLS CO-TRANSFORMED BY HPV-16 PLUS EJ-RAS
CONTINUED EXPRESSION OF HPV-16 E7 PROTEIN IS REQUIRED FOR MAINTENANCE OF THE TRANSFORMED PHENOTYPE OF CELLS CO-TRANSFORMED BY HPV-16 PLUS EJ-RAS
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DOI：
10.1002/j.1460-2075.1989.tb03405.x
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发表时间：
1989-02-01
期刊：
影响因子：
11.4
通讯作者：
BANKS, L
中科院分区：
文献类型：
--
作者：
CROOK, T;MORGENSTERN, JP;BANKS, L
HPV-16与宫颈癌之间的密切联系意味着病毒在这种癌症的发展中发挥了一定的作用。最近的研究表明，HPV-16E7基因编码了病毒的主要转化活性，在乳鼠肾脏(BRK)细胞转化实验中。为了研究HPV-16E7+EJ-ras转化的BRK细胞对E7持续表达的要求，我们建立了E7基因的诱导表达系统。本文报道的研究表明，HPV-16E7基因的持续表达是维持这些细胞转化表型所必需的。这些观察结果对E7基因在宫颈癌中的作用进行了讨论。
The close association between HPV-16 and cervical cancer implies some role for the virus in development of this cancer. Recent studies have shown that the HPV-16 E7 gene encodes the major transforming activity of the virus in baby rat kidney (BRK) cell transformation assays. To investigate the requirement for continued E7 expression in BRK cells transformed by HPV-16 E7 plus EJ-ras, we have developed a system for inducible expression of the E7 gene. The studies reported here show that continued expression of the HPV-16 E7 gene is required for maintenance of the transformed phenotype in these cells. The implications these observations bear on the role of the E7 gene in cervical carcinoma are discussed.