Amino acid sequence of a basic blue protein from cucumber seedlings.
Amino acid sequence of a basic blue protein from cucumber seedlings.
复制标题
黄瓜幼苗碱性蓝色蛋白的氨基酸序列。
DOI：
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发表时间：
1982
影响因子：
11.1
通讯作者：
F. Morgan
中科院分区：
文献类型：
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作者：
Mitsuo Murata;G. Begg;F. Lambrou;B. Leslie;Richard J. Simpson;H. C. Freeman;F. Morgan
1 型铜蛋白（来自黄瓜幼苗的 96 个残基的碱性蓝色蛋白）的氨基酸序列已通过对完整分子以及用溴化氰和胰蛋白酶切割产生的片段进行 Edman 降解来确定。黄瓜碱性蓝色蛋白与星花青蛋白具有显着的序列同源性，与质体蓝蛋白和天青蛋白具有较小程度的同源性。已知的质体蓝蛋白和天青蛋白的铜配体（对应于质体蓝蛋白中的组氨酸 37、半胱氨酸 84、组氨酸 87 和蛋氨酸 92）存在于黄瓜碱性蓝色蛋白中。然而，后者还含有一个半胱氨酸残基，类似于建议的星花青蛋白第四个配体，其中不存在蛋氨酸。
The amino acid sequence of a type 1 copper protein, the 96-residue basic blue protein from cucumber seedlings, has been determined by Edman degradation of the intact molecule and of fragments produced by cleavage with cyanogen bromide and with trypsin. The cucumber basic blue protein shows a marked sequence homology with stellacyanin, and to a smaller degree with plastocyanin and azurin. The known copper ligands of plastocyanin and azurin (corresponding to histidine-37, cysteine-84, histidine-87, and methionine-92 in plastocyanin) are present in the cucumber basic blue protein. However, the latter also contains a half-cystine residue analogous to the suggested fourth ligand of stellacyanin, where methionine is absent.