A roadmap for biocatalysis - functional and spatial orchestration of enzyme cascades.
A roadmap for biocatalysis - functional and spatial orchestration of enzyme cascades.
复制标题
生物催化的路线图 - 酶级联的功能和空间编排。
DOI：
10.1111/1751-7915.12386
复制
发表时间：
2016-09
影响因子：
5.7
通讯作者：
Lopez-Gallego F
中科院分区：
文献类型：
--
作者：
Schmidt-Dannert C;Lopez-Gallego F
生物工程和系统生物学的发展为生物产业化提供了新的途径和工具。在未来十年中，先进的生物催化系统将越来越多地用于生产目前工艺无法生产的化学品和/或使用酶催化剂的资源效率更高，对环境的影响大大减少。我们预计，在未来，化学品和材料的生产将利用生物催化和化学合成协同。实现这种先进的生物制造工艺目前面临着许多重大挑战。用于设计到生产的可部署生物催化剂组合必须从生物多样性来源和通过蛋白质工程创建。必须开发出强大而高效的多步酶促反应级联，这些级联可以在一锅中同时操作。为此，必须开发生物催化剂活动的空间和时间控制的生物正交策略。有前途的方法和技术正在出现，最终将导致体外生物催化系统的设计，模拟代谢途径和网络的细胞系统，这将在本路线图中讨论。
Advances in biological engineering and systems biology have provided new approaches and tools for the industrialization of biology. In the next decade, advanced biocatalytic systems will increasingly be used for the production of chemicals that cannot be made by current processes and/or where the use of enzyme catalysts is more resource efficient with a much reduced environmental impact. We expect that in the future, manufacture of chemicals and materials will utilize both biocatalytic and chemical synthesis synergistically. The realization of such advanced biomanufacturing processes currently faces a number of major challenges. Ready‐to‐deploy portfolios of biocatalysts for design to production must be created from biological diverse sources and through protein engineering. Robust and efficient multi‐step enzymatic reaction cascades must be developed that can operate simultaneously in one‐pot. For this to happen, bio‐orthogonal strategies for spatial and temporal control of biocatalyst activities must be developed. Promising approaches and technologies are emerging that will eventually lead to the design of in vitro biocatalytic systems that mimic the metabolic pathways and networks of cellular systems which will be discussed in this roadmap.