Analysis of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum by multilocus enzyme electrophoresis
Analysis of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum by multilocus enzyme electrophoresis
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猪红斑丹毒丝菌和扁桃体丹毒丝菌的多位点酶电泳分析
DOI：
10.1128/jcm.32.2.371-376.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
M J Turner
中科院分区：
文献类型：
--
作者：
K. N. Chooromoney;D. J. Hampson;G. Eamens;M J Turner
对74株猪丹毒丝菌澳大利亚分离株和22株猪丹毒丝菌血清型参考株的遗传多样性进行了研究。采用多位点酶电泳法检测扁桃体红斑病或丹毒丝菌。4株E. tonsillarum（血清型25、3、10和22的菌株KS 20 A、Wittling、Lengyel-P和Bano 107）与E.红斑病然而，E.扁桃体14型参考菌株（Iszap-4）和13型参考菌株（佩奇-56）被认为代表了一个新的基因组种，它们与大肠杆菌的典型分离株和参考菌株聚在一起。红斑病我们的血清型7的参考菌株（Rotzunge）也是E. rhusiopathiae，因此，这些血清型反应不能依赖于作为一种手段，确定分离株作为E。扁桃体澳大利亚E. rhusiopathiae是遗传多样性。从绵羊或鸟类中回收的菌株比从猪中分离的菌株更多样，血清型1的菌株比血清型2的菌株更多样。同一血清型分离株之间的差异以及同一电泳型中不同血清型分离株的存在表明，E.用红斑狼疮作为流行病学工具不可靠。一些样本含有从不同动物种属中回收的分离株。ET-41菌株对两株参考菌株的耐药率为32.2%，表明该菌株对大肠杆菌的耐药率较高。红斑病在各种动物中广泛存在并且通常与疾病相关。
The genetic diversity of 74 Australian field isolates of Erysipelothrix rhusiopathiae and 22 reference strains for serovars of E. rhusiopathiae or Erysipelothrix tonsillarum was examined by multilocus enzyme electrophoresis. Four serovar reference strains of E. tonsillarum (strains KS 20 A, Wittling, Lengyel-P, and Bano 107 for serovars 25, 3, 10, and 22, respectively) were genetically distinct from E. rhusiopathiae. However, the E. tonsillarum reference strain for serovar 14 (Iszap-4) and the reference strain for serovar 13 (Pecs-56), which has been said to represent a new genomic species, were found to cluster with typical isolates and reference strains of E. rhusiopathiae. Our reference strain for serovar 7 (Rotzunge) was also genetically typical of E. rhusiopathiae, thus indicating that these serotype reactivities cannot be relied upon as a means of identifying isolates as E. tonsillarum. Australian field isolates of E. rhusiopathiae were genetically diverse. Those recovered from sheep or birds were more diverse than those isolated from pigs, and isolates of serovar 1 were more diverse than those of serovar 2. The diversity found among isolates of the same serovar and the presence of isolates of different serovars in the same electrophoretic types (ETs) indicated that serotyping of E. rhusiopathiae was unreliable for use as an epidemiological tool. Some ETs contained isolates recovered from different animal species. ET 41 contained 32.2% of the field isolates and two reference strains, indicating that this clone of E. rhusiopathiae is both widespread and commonly associated with disease in various species of animals.