Glaucoma Drainage Device Coated with Mitomycin C Loaded Opal Shale Microparticles to Inhibit Bleb Fibrosis.
Glaucoma Drainage Device Coated with Mitomycin C Loaded Opal Shale Microparticles to Inhibit Bleb Fibrosis.
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DOI：
10.1021/acsami.8b18551
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发表时间：
2019-01
影响因子：
9.5
通讯作者：
Aimeng Dong;Liang Han;Zhengbo Shao;Pan Fan;Xin-rong Zhou;Huiping Yuan
中科院分区：
文献类型：
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作者：
Aimeng Dong;Liang Han;Zhengbo Shao;Pan Fan;Xin-rong Zhou;Huiping Yuan
过度的纤维化是青光眼引流器植入失败的首要因素。辅助药物方法有望帮助减少瘢痕形成和过度纤维化。蛋白石页岩是一种天然状态的非晶态硅质物质，具有多孔性和强吸附性，极有可能用于药物的载药和缓释。在这里，我们采用超声波和离心的OS微粒（MP），并提出了一种创新的和改进的GDD与OS MP包被，其中载有丝裂霉素C（MMC）。MMC负载的OS MP通过OS的吸附性被物理吸收在Ahmed青光眼阀表面上。在改良的Ahmed青光眼阀上负载MMC约5.51 μg，体外可持续释放18天。MMC加载的OS MP抑制成纤维细胞增殖，并显示出对原代Tenon's成纤维细胞的低毒性。改良引流装置耐受性良好，可有效减少体内纤维反应。因此，我们的研究使用OS MP构建了一种改进的Ahmed青光眼阀，而不会干扰阀表面上的房水引流模式。改良Ahmed青光眼阀成功缓解了GDD植入手术后的瘢痕组织形成。
Excessive fibrosis is the topmost factor for the defeat of surgical glaucoma drainage device (GDD) implantation. Adjuvant drug approaches are promising to help reduce the scar formation and excessive fibrosis. Opal shale (OS), as a natural state and noncrystalline silica substance with poriferous nature and strong adsorbability, is highly likely to undertake drug loading and delivery. Here, we employed OS microparticles (MPs) by ultrasound and centrifugation and presented an innovative and improved GDD coated with OS MPs, which were loaded with mitomycin C (MMC). MMC-loaded OS MPs were physically absorbed on the Ahmed glaucoma valve surface through OS' adsorbability. About 5.51 μg of MMC was loaded on the modified Ahmed glaucoma valve and can be released for 18 days in vitro. MMC-loaded OS MPs inhibited fibroblast proliferation and showed low toxicity to primary Tenon's fibroblasts. The ameliorated drainage device was well tolerated and effective in reducing the fibrous reaction in vivo. Hence, our study constructed an improved Ahmed glaucoma valve using OS MPs without disturbing aqueous humor drainage pattern over the valve surface. The modified Ahmed glaucoma valve successfully alleviated scar tissue formation after GDD implantation surgery.