Tryptophan autofluorescence imaging of neoplasms of the human colon
Tryptophan autofluorescence imaging of neoplasms of the human colon
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DOI：
10.1117/1.jbo.17.1.016003
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Utzinger, Urs
中科院分区：
文献类型：
--
作者：
Banerjee, Bhaskar;Renkoski, Timothy;Utzinger, Urs
扁平瘤变的检测是结直肠癌筛查的主要挑战，因为遗漏的病变可能导致在随后的内窥镜检查之前发生意外的“意外”癌症。据报道，色氨酸相关自身荧光的使用在小鼠肠道发育不良中增加。研究了从人腺癌和正常结肠粘膜中分离的细胞的发射光谱，发现癌细胞的发射强度明显高于从周围正常粘膜中获得的细胞。采用一种优化组织紫外宏观成像的多光谱成像系统原型，获得结肠肿瘤手术标本和正常粘膜切除后的自体荧光图像。与正常粘膜相比，荧光图像没有显示预期的肿瘤更大的发射，很可能是由于肿瘤的光吸收和散射增加。然而，一旦用反射图像校正荧光图像，观察到肿瘤的荧光强度增加。色氨酸单独荧光可用于区分正常细胞和癌细胞，而在组织中，其自身荧光图像被绿色反射分割可用于显示肿瘤。(c) 2012年中国光学仪器工程师学会（SPIE）；(jbo.17.1.016003 DOI: 10.1117/1.)
Detection of flat neoplasia is a major challenge in colorectal cancer screening, as missed lesions can lead to the development of an unexpected 'incident' cancer prior to the subsequent endoscopy. The use of a tryptophan-related autofluorescence has been reported to be increased in murine intestinal dysplasia. The emission spectra of cells isolated from human adenocarcinoma and normal mucosa of the colon were studied and showed markedly greater emission intensity from cancerous cells compared to cells obtained from the surrounding normal mucosa. A proto-type multispectral imaging system optimized for ultraviolet macroscopic imaging of tissue was used to obtain autofluorescence images of surgical specimens of colonic neoplasms and normal mucosa after resection. Fluorescence images did not display the expected greater emission from the tumor as compared to the normal mucosa, most probably due to increased optical absorption and scattering in the tumors. Increased fluorescence intensity in neoplasms was observed however, once fluorescence images were corrected using reflectance images. Tryptophan fluorescence alone may be useful in differentiating normal and cancerous cells, while in tissues its autofluorescence image divided by green reflectance may be useful in displaying neoplasms. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.1.016003]