An abundance of bidirectional promoters in the human genome
An abundance of bidirectional promoters in the human genome
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DOI：
10.1101/gr.1982804
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发表时间：
2004-01-01
期刊：
影响因子：
7
通讯作者：
Myers, RM
中科院分区：
文献类型：
--
作者：
Trinklein, ND;Aldred, SF;Myers, RM
全长人类cDNA序列与人类基因组的完成序列的比对为研究基因在整个基因组中的分布提供了独特的机会。通过分析23，752个基因之间的距离，我们确定了一类转录差异的基因对，占基因组中超过10%的基因，其转录起始位点之间的间隔小于1000个碱基对。虽然这种双向排列在人类和其他物种中已经被描述过，但人类基因组中双向基因对的普遍性是惊人的，并且除了少数双向基因之外，所有双向基因的调控机制都是未知的。我们的工作表明，许多双向对的转录物是共表达的，但有些是反调节的。此外，我们发现，两个双向基因之间的许多启动子片段启动两个方向的转录，并包含共同的元件，调节两个基因。我们还表明，双向排列往往是保守的小鼠直系同源物。这些发现表明，一个双向的安排提供了一个独特的机制，大量的哺乳动物基因的调控。
The alignment of full-length human cDNA sequences to the finished sequence of the human genome provides a unique opportunity to study the distribution of genes throughout the genome. By analyzing the distances between 23,752 genes, we identified a class of divergently transcribed gene pairs, representing more than 10% of the genes in the genome, whose transcription start sites are separated by less than 1000 base pairs. Although this bidirectional arrangement has been previously described in humans and other species, the prevalence of bidirectional gene pairs in the human genome is striking, and the mechanisms of regulation of all but a few bidirectional genes are unknown. Our work shows that the transcripts of many bidirectional pairs are coexpressed, but some are antiregulated. Further, we show that many of the promoter segments between two bidirectional genes initiate transcription in both directions and contain shared elements that regulate both genes. We also show that the bidirectional arrangement is often conserved among mouse orthologs. These findings demonstrate that a bidirectional arrangement provides a unique mechanism of regulation for a significant number of mammalian genes.