Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement
Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement
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DOI：
10.1016/j.bios.2010.07.038
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发表时间：
2010-12-15
影响因子：
12.6
通讯作者：
Lee, Won-Yong
中科院分区：
文献类型：
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作者：
Min, In-Hong;Choi, Lee;Lee, Won-Yong
首次使用碳水化合物稳定的金纳米颗粒和银增强技术开发了一种高灵敏度电化学凝集素生物传感器。目标凝集素蛋白伴刀豆球蛋白 A (Con A) 与金电极上自组装的硫醇化甘露糖单层特异性结合。添加甘露糖稳定的金纳米粒子，与Con A形成夹心型复合物，然后通过银增强工艺，用银金属涂覆甘露糖稳定的金纳米粒子。将涂覆的金属银溶解在酸性溶液中，并通过阳极溶出伏安法检测所得的银离子。本凝集素生物传感器对Con A浓度从0.084 μg/mL到50.0 μg/mL给出了线性响应(R-2 = 0.999)，检测限(S/N = 3)为0.070 μg/mL，比报道的微重和比色检测方法获得的检测限低得多。 (C) 2010 Elsevier B.V. 保留所有权利。
A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R-2 = 0.999) for Con A concentration from 0.084 mu g/mL to 50.0 mu g/mL with a remarkable detection limit (S/N = 3) of 0.070 mu g/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods. (C) 2010 Elsevier B.V. All rights reserved.