Genetic characterization of hantaviruses isolated from Guizhou, China: Evidence for spillover and reassortment in nature
Genetic characterization of hantaviruses isolated from Guizhou, China: Evidence for spillover and reassortment in nature
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DOI：
10.1002/jmv.21149
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发表时间：
2008-06-01
影响因子：
12.7
通讯作者：
Zhang, Yong-Zhen
中科院分区：
文献类型：
--
作者：
Zou, Yang;Hu, Jing;Zhang, Yong-Zhen
为了更深入地了解汉坦病毒在贵州省的流行病学，2001年至2003年期间，在贵州省捕获啮齿动物。此外，从1例患者中采集血清样本。尝试从人血清和啮齿动物样品中分离病毒。在细胞培养中成功地从一个人、两个A. agrarius和R.挪威人。测定了这4个分离株和20世纪80年代分离的6个病毒的全部S、M和部分L片段的核苷酸序列。系统发育分析表明，所有分离株的S片段都属于汉滩病毒（HTNV）分支，无论它们来自何处。根据S序列，这些病毒可分为三个不同的类群，显示地理聚集性。整个M和部分L片段序列的分析表明，8的10个分离物属于HTNV进化枝。而从R.褐家鼠属于首尔病毒（SEOV）分支。此外，这两个分离物不同于SEOV进化枝的其他已知成员。总之，这些数据表明，至少有三组HTNV是共循环的，一种新的SEOV变体可能存在于贵州。我们的研究结果还表明，HTNV从A。agrarius溢出到R. HTNV和SEOV之间的自然重配发生在溢出期间或之后。
To gain more insights into the epidemiology of hantaviruses in the Guizhou province, China, rodents were captured in Guizhou during the period from 2001 to 2003. In addition, serum sample was collected from one patient. Virus isolation was attempted from human serum and rodent samples. Four hantaviruses were isolated successfully in cell culture from one human, two A. agrarius, and one R. norvegicus. The nucleotide sequences for the entire S and M and partial L segment were determined from these four isolates as well as six viruses isolated in 1980s. Phylogenetic analysis revealed that the S segment from all isolates belong to the Hantaan virus (HTNV) clade, regardless of the sources from which they were derived. According to the S sequences, these viruses could be divided into three distinct phylogroups, showing geographical clustering. Analysis of the entire M and the partial L segment sequences demonstrated that 8 out of the 10 isolates belong to the HTNV clade. However, two isolates (CGRn8316 and CGRn9415) isolated from R. norvegicus belong to the Seoul virus (SEOV) clade. In addition, these two isolates were distinct from other known members of SEOV clade. Together, the data suggest that at least three groups of HTNV are co-circulating and one new variant of SEOV may be present in Guizhou. Our results also suggest that HTNV from A. agrarius spilled over to R. norvegicus and natural reassortment between HTNV and SEOV occurred during or after the spillover.