Anti-Mullerian hormone inhibits initiation of growth of human primordial ovarian follicles in vitro
Anti-Mullerian hormone inhibits initiation of growth of human primordial ovarian follicles in vitro
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DOI：
10.1093/humrep/del165
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发表时间：
2006-09-01
影响因子：
6.1
通讯作者：
Hovatta, O.
中科院分区：
文献类型：
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作者：
Carlsson, I. B.;Scott, J. E.;Hovatta, O.
背景：抗苗勒管激素（AMH）抑制小鼠卵泡的发育和早期生长。此外，AMH基因敲除小鼠的卵泡池过早减少。在这项研究中，我们研究了AMH是否在人类中发挥类似的作用，控制卵泡生长。方法：将人卵巢皮质组织活检标本切成小块，并在含有0、10、30或100 ng/ml大鼠重组AMH的培养基中培养7天。从组织学切片评价卵泡的发育阶段和活力。研究结果：与之前的研究相似，几乎在所有培养基中均观察到卵泡生长的显著启动，与未培养的对照组织（56%）相比，原始卵泡（14-26%）的比例显著较小。例外的是补充有100 ng/ml AMH的培养基中的组织。在此，原始卵泡的比例与非培养组织中的原始卵泡的比例无显著差异;此外，原始卵泡的比例显著高于溶剂对照培养物和含10 ng/ml AMH的培养物中的原始卵泡的比例，表明生长起始受到抑制。与对照培养基中的组织相比，AMH的存在不影响活力。结论：100 ng/ml的重组AMH在体外对人早期卵泡发育具有抑制作用，抑制原始卵泡生长的起始。
BACKGROUND: Anti-Mullerian hormone (AMH) inhibits the initiation of the development and early growth of mouse ovarian follicles. Furthermore, the ovarian follicle pool diminishes prematurely in AMH-knockout mice. In this study, we examined whether AMH plays a similar role in humans, controlling ovarian follicle growth. METHODS: Human ovarian cortical tissue biopsy specimens were cut into small pieces and cultured for 7 days in medium containing rat recombinant AMH at 0, 10, 30 or 100 ng/ml. The developmental stages and viability of the follicles were evaluated from histological sections. RESULTS: Similar to previous studies, significant initiation of follicle growth was observed in almost all culture media, as demonstrated by a significantly smaller proportion of primordial follicles (14-26%) compared with non-cultured control tissue (56%). The exception was tissue in medium supplemented with AMH at 100 ng/ml. Here, the proportion of primordial follicles was not significantly different from that in non-cultured tissue; furthermore, it was significantly greater than that in vehicle control cultures and cultures containing AMH at 10 ng/ml, indicating the inhibition of growth initiation. Viability was unaffected by the presence of AMH when compared with tissues in control media. CONCLUSIONS: Recombinant AMH at a concentration of 100 ng/ml has an inhibitory effect on early human ovarian follicular development in vitro, suppressing the initiation of primordial follicle growth.