Genome-Wide Expression Analysis of Soybean MADS Genes Showing Potential Function in the Seed Development
Genome-Wide Expression Analysis of Soybean MADS Genes Showing Potential Function in the Seed Development
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大豆 MADS 基因的全基因组表达分析显示在种子发育中的潜在功能
DOI：
10.1371/journal.pone.0062288
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发表时间：
2013-04-30
期刊：
影响因子：
3.7
通讯作者：
Fu, Yong-Fu
中科院分区：
文献类型：
--
作者：
Fan, Cheng-Ming;Wang, Xu;Fu, Yong-Fu
MADS家族是一个古老且研究最多的转录因子，在植物的几乎所有发育过程中都起着重要作用。在植物进化史上，全基因组复制（WGD）事件不仅对植物物种的进化，而且对基因家族成员的扩展都具有重要意义。大豆作为模式豆类作物，经历了三轮WGD事件。在大豆3轮WGD事件后，一些MIKCC亚家族的成员，如SOC、AGL 6、SQUA、SVP、AGL 17和DEF/GLO都得到了扩增。而一些MIKCC亚家族、MIKC* 和I型MADS家族在Glycine WGD事件之前经历了较快的生与死进化，未发现它们的踪迹，转座重复在不同亚家族成员之间的串联排列中发挥着重要作用。根据I型和MIKC副作用基因对的表达谱，MIKC副作用基因对的命运为亚功能化，而I型MADS副作用基因对的命运为非功能化。在163个MADS基因中，有137个与种子相关QTL的2 Mb基因组区域内的186个位点接近，其中115个基因在种子发育过程中表达。虽然MIKCC基因在花发育中具有重要的保守功能，但大多数MIKCC基因在种子发育和I型MADS中也具有潜在的重要作用。
The MADS family is an ancient and best-studied transcription factor and plays fundamental roles in almost every developmental process in plants. In the plant evolutionary history, the whole genome duplication (WGD) events are important not only to the plant species evolution, but to expansion of members of the gene families. Soybean as a model legume crop has experience three rounds of WGD events. Members of some MIKCC subfamilies, such as SOC, AGL6, SQUA, SVP, AGL17 and DEF/GLO, were expanded after soybean three rounds of WGD events. And some MIKCC subfamilies, MIKC* and type I MADS families had experienced faster birth-and-death evolution and their traces before the Glycine WGD event were not found. Transposed duplication played important roles in tandem arrangements among the members of different subfamilies. According to the expression profiles of type I and MIKC paralog pair genes, the fates of MIKC paralog gene pairs were subfunctionalization, and the fates of type I MADS paralog gene pairs were nonfunctionalization. 137 out of 163 MADS genes were close to 186 loci within 2 Mb genomic regions associated with seed-relative QTLs, among which 115 genes expressed during the seed development. Although MIKCC genes kept the important and conserved functions of the flower development, most MIKCC genes showed potentially essential roles in the seed development as well as the type I MADS.