Phosphorus-31 nuclear magnetic resonance studies of single muscle cells isolated from barnacle depressor muscle.
Phosphorus-31 nuclear magnetic resonance studies of single muscle cells isolated from barnacle depressor muscle.
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DOI：
10.1021/bi00283a035
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发表时间：
1983-07
期刊：
影响因子：
2.9
通讯作者：
G. Dubyak;A. Scarpa
中科院分区：
文献类型：
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作者：
G. Dubyak;A. Scarpa
George R. Dubyak* 和 Antonio Scarpa 摘要：从藤壶 Balanusnubilis 的下压肌中分离出的单个肌肉细胞记录了 31P NMR 谱 (145.7 MHz)。单细胞安装在小直径（2.2 毫米）的螺线管接收线圈中，使用 45 个脉冲进行扫描，延迟时间范围为 1 至 10 秒； 400 次扫描（总累积时间为 10-15 分钟）足以获得具有良好信噪比的光谱。解剖后 30 分钟内获得的光谱显示，磷酸精氨酸和 ATP 是静息条件下这些细胞中唯一以明显 (> 1 mM) 浓度存在的磷酸代谢物。随着解剖后时间的增加，磷酸精氨酸含量降低 6-8%/h。这种消耗与糖磷酸酯和无机磷酸盐浓度的伴随增加相匹配；在这些变化期间 ATP 保持在恒定水平
George R. Dubyak* and Antonio Scarpa abstract: 31P NMR spectra (145.7 MHz) were recorded from single muscle cells isolated from the depressor muscle of the barnacle Balanusnubilis. The single cells, mounted in a small (2.2-mm) diameter solenoidal receiver coil, were scanned by using 45 pulses with delay times ranging from 1 to 10 s; 400 scans (10-15-min total accumulation time) were sufficient to obtain spectra with good signal/noise ratios. Spectra obtained within 30 min after dissectionreveal that phosphoarginine and ATP are the only phosphometabolites present in appreciable (> 1 mM) concentration in these cells under resting conditions. As the postdissection time is in-creased, the phosphoarginine content decreases 6-8%/h. This depletion is matched by concomitant increases in the con-centrations of sugar phosphate esters and inorganic phosphate; ATP is maintained at constant levels during these shifts in