A modular cross-linking approach for exploring protein interactions
A modular cross-linking approach for exploring protein interactions
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DOI：
10.1021/ja026917a
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发表时间：
2003-03-05
影响因子：
15
通讯作者：
Muir, TW
中科院分区：
文献类型：
--
作者：
Trester-Zedlitz, M;Kamada, K;Muir, TW
描述了一种使用新型交联剂和质谱法阐明蛋白质-蛋白质相互作用的方法。该方法包括（1）用于生成交联试剂的模块化固相合成策略，（2）使用微量浓缩器富集和消化交联蛋白质，（3）交联肽的质谱分析，以及（4）交联数据的综合计算分析。这种集成的方法已被应用到异二聚体蛋白质复合物负辅因子2的组分之间的交联的研究。
A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.