AND-Logic Strategy for Accurate Analysis of Alzheimer's Disease via Fluorescent Probe Lighted Up by Two Specific Biomarkers
AND-Logic Strategy for Accurate Analysis of Alzheimer's Disease via Fluorescent Probe Lighted Up by Two Specific Biomarkers
复制标题
通过两种特定生物标记物点亮的荧光探针准确分析阿尔茨海默病的 AND 逻辑策略
DOI：
10.1021/acs.analchem.1c02943
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发表时间：
2021
影响因子：
7.4
通讯作者：
Ding Caifeng
中科院分区：
文献类型：
--
作者：
Zhang Peng;Fu Caixia;Liu Haihong;Guo Xinjie;Zhang Qian;Gao Jian;Chen Wenjuan;Yuan Wei;Ding Caifeng
阿尔茨海默病（AD）已成为全球老年人健康的威胁，诊断后生存时间短。由于早期发展无症状，患者通常在中晚期才被诊断出来。因此，一种有效的 AD 早期诊断工具值得关注，这可以为治疗干预做出重大贡献。荧光探针已广泛应用于体外和体内检测和可视化感兴趣的物种，探针和分析物之间的适当反应负责荧光变化，以提供具有令人满意的传感行为的照明或比率响应模式。在这项工作中，我们首次尝试建立一个 AND 逻辑探针 P2，以丁酰胆碱酯酶 (BChE) 和活性氧 (ROS) 作为双重目标进行 AD 准确诊断。在这两个因素通过酶水解和氧化还原反应共同刺激时，可以很容易地开启近红外发射。这种 AND 传感模式有效地避免了假阳性反应，并且共享一种生物标志物的其他疾病很难诱导 NIR 荧光反应。该传感检测方法在体外和体内也被证实是可行的，具有良好的灵敏度和选择性。值得一提的是，探针结构在连接长度方面进行了优化。研究表明，具有中等长度连接臂（一个亚甲基，n=1）的探针P2具有优越的传感性能，有望为相关结构设计提供参考。
Alzheimer’s disease (AD) has become a global threat to the elderly health with a short survival time after diagnosis. Due to the asymptomatic stage during the early development, patients are usually diagnosed at the middle or late stage. Therefore, an efficient tool for AD early diagnosis deserves considerable attention, which could make a significant contribution to the treatment intervention. A fluorescent probe has been widely applied for detecting and visualizing species of interestin vitroandin vivo, and the proper reaction between the probe and analytes is responsible for the fluorescence change to provide a lighting-on or ratiometric responsive pattern with satisfactory sensing behavior. In this work, we report the first attempt to build up an AND-logic probeP2for AD accuracy diagnosis taking butyrylcholinesterase (BChE) and reactive oxygen species (ROSs) as dual targets. Upon the co-stimulation by these two factors through enzymatic hydrolysis and redox reaction, the NIR emission could be readily turned on. This AND sensing pattern avoided the false-positive response effectively, and other diseases sharing one biomarker could hardly induce a NIR fluorescence response. The sensing assay has also been confirmed to be feasiblein vitroandin vivowith good sensibility and selectivity. It is worth mentioning that the probe structure has been optimized in terms of the linkage length. This study shows that probeP2with a connecting arm of medium length (one methylene,n= 1) has superior sensing performance, promising to provide a reference for the relative structure design.