Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus)
Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus)
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尼罗罗非鱼 (Oreochromis niloticus) 的 CD28 和 CD80/86 分子的结构和相互作用特征的保护
DOI：
10.1016/j.fsi.2017.10.008
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Jian, Jichang
中科院分区：
文献类型：
--
作者：
Huang, Yu;Wang, Zhiwen;Jian, Jichang
CD 28与CD 80或CD 86分子的相互作用提供了T细胞活化所需的共刺激信号。本研究从尼罗罗非鱼（Oreochromis niloticus）中克隆并分析了CD 28基因（On-CD 28）和CD 80/86基因（On-CD 80/86）。序列分析揭示了On-CD 28蛋白的典型特征;例如，基于脯氨酸的基序（（TYPPPL 122）-T-117）在CD 28与CD 80/86配体的结合中是必需的。此外，在On-CD 80/86中发现了细胞外IG结构域;该结构域负责CD 28与CD 80/86受体的结合。亚细胞定位分析显示On-CD 28和On-CD 80/86主要分布在细胞膜上。酵母双杂交实验表明On-CD 28与On-CD 80/86直接相互作用。On-CD 28和On-CD 80/86在健康尼罗罗非鱼的所有组织中均有表达，其中On-CD 28和On-CD 80/86在脑和心脏中的表达量最高。在体内使用无乳链球菌进行细菌攻击后，On-CD 28和On-CD 80/86在头肾、脾、肠和脑中上调。然而，它们对灭活S.体外无乳。这些结果表明On-CD 28与On-CD 80/86的相互作用提供了一种共刺激信号，可能在S.无乳感染
Interaction of CD28 with CD80 or CD86 molecules provides a costimulatory signals required in T cell activation. In this study, we cloned and analyzed a CD28 gene (On-CD28) and a CD80/86 gene (On-CD80/86) from Nile tilapia (Oreochromis niloticus). Sequence analysis revealed the typical characteristics of On-CD28 protein; for instance, the proline-based motif ((TYPPPL122)-T-117) is essential in binding of CD28 to CD80/86 ligands. Moreover, an extracellular Ig domain was found in On-CD80/86; this domain is responsible in binding of CD28 to CD80/86 receptors. Subcellular localization analysis showed that both On-CD28 and On-CD80/86 were distributed predominantly in the cytomembrane. Yeast two-hybrid assay showed that On-CD28 directly interacted with On-CD80/86. On-CD28 and On-CD80/86 transcripts were detected in all the examined tissues of healthy Nile tilapia, and the highest expression levels of On-CD28 and On-CD80/86 were detected in the brain and heart, respectively. Following a bacterial challenge using Streptococcus agalactiae in vivo, On-CD28 and On-CD80/86 were upregulated in head kidney, spleen, intestines, and brain. However, they showed different expression profiles in response to stimulation with inactivated S. agalactiae in vitro. These findings indicated that the interaction of On-CD28 with On-CD80/86 provides a costimulatory signals that possibly play an important role in T cell activation during S. agalactiae infection.