ASSESSMENT OF ELASTASE AS A PSEUDOMONAS-AERUGINOSA VIRULENCE FACTOR IN EXPERIMENTAL LUNG INFECTION IN MINK
ASSESSMENT OF ELASTASE AS A PSEUDOMONAS-AERUGINOSA VIRULENCE FACTOR IN EXPERIMENTAL LUNG INFECTION IN MINK
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DOI：
10.1016/0378-1135(87)90090-3
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发表时间：
1987-03-01
影响因子：
3.3
通讯作者：
IGLEWSKI, BH
中科院分区：
文献类型：
--
作者：
ELSHEIKH, LE;KRONEVI, T;IGLEWSKI, BH
将一株产弹性蛋白酶的铜绿假单胞菌(PAO1)和两株弹性酶形成缺陷的突变株(PAO1-E64和PAO1-LAS-16)接种于水貂的气管内。感染突变株的水貂存活时间延长，肺组织镜检显示，弹性酶阳性野生型菌株比弹性酶缺陷突变株更能引起组织损伤和出血。PAO1和PAO1-LAS-16菌株还与3株产弹性酶不同的水貂自然感染菌株进行了比较。与其他品系相比，具有高或中等弹性酶产量的水貂品系会产生更严重的肺损伤，并与更高的死亡率相关。结果表明，铜绿假单胞菌弹性酶可增强肺部感染时该菌的毒力。
Anaethesized mink were inoculated intratracheally with an elastase-producing Pseudomonas aeruginosa strain (PAO1) and two mutants derived from PAO1 with defective elastase formation (strains PAO1-E64 and PAO1-las-16). Survival times were prolonged in mink infected with the mutants, and microscopic examination of lung showed that the elastase-positive wild type strain produced more pronounced tissue damage and haemorrhages than did the elastase-defective mutant strains. The strains PAO1 and PAO1-las-16 were also compared to three strains isolated from natural infection in mink which differed in elastase production. The mink strains with high or moderate elastase production produce more severe lung damage and were associated with a higher mortality than the other strains tested. The results indicate that P. aeruginosa elastase may enhance the virulence of the bacterium in lung infections.