Interferon-γ induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases
Interferon-γ induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases
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DOI：
10.1017/s0031182099005521
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发表时间：
2000-03-01
期刊：
影响因子：
2.4
通讯作者：
Harris, RA
中科院分区：
文献类型：
--
作者：
Hamadien, M;Bakhiet, M;Harris, RA
Bidirectional signalling between Trypanosoma brucei brucei and CD8(+) T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.
