Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01
Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01
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DOI：
10.1016/j.biortech.2011.05.068
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发表时间：
2011-09-01
影响因子：
11.4
通讯作者：
Chen, Guo-Qiang
中科院分区：
文献类型：
--
作者：
Tan, Dan;Xue, Yuan-Sheng;Chen, Guo-Qiang
以从新疆某盐湖中分离到的一株嗜盐菌--盐单胞菌TD 01为出发菌株，建立了一种非灭菌连续发酵工艺。该菌株在葡萄糖盐培养基上经过56 h的分批补料培养，细胞干重达到80 g/L，其中聚（3-羟基丁酸酯）含量为80%。在14天的开放非无菌和连续的过程中，细胞生长到平均40 g/L的细胞干重含有60%的聚羟基丁酸在第一发酵罐与葡萄糖盐培养基。将培养物从第一个发酵罐连续泵入含有缺氮葡萄糖盐培养基的第二个发酵罐，稀释了细胞，但使它们的PHB水平维持在细胞干重的65%至70%之间。在第一个发酵罐中葡萄糖转化为PHB的转化率在20%和30%之间，在第二个发酵罐中高于50%。这种非无菌连续发酵工艺为降低聚羟基链烷酸酯生产成本开辟了新的领域。(C)2011爱思唯尔有限公司保留所有权利。
An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56 h fed-batch process. In a 14-day open unsterile and continuous process, the cells grew to an average of 40 g/L cell dry weight containing 60% PHB in the first fermentor with glucose salt medium. Continuous pumping of cultures from the first fermentor to the second fermentor containing the nitrogen-deficient glucose salt medium diluted the cells but allowed them to maintain a PHB level of between 65% and 70% of cell dry weight. Glucose to PHB conversions were between 20% and 30% in the first fermentor and above 50% in the second one. This unsterile and continuous fermentation process opens a new area for reducing the cost in polyhydroxyalkanoates production. (C) 2011 Elsevier Ltd. All rights reserved.