Prolonged survival of mouse skin allografts in recipients treated with donor splenocytes and antibody to CD40 ligand
Prolonged survival of mouse skin allografts in recipients treated with donor splenocytes and antibody to CD40 ligand
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DOI：
10.1097/00007890-199707270-00026
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发表时间：
1997-07-27
期刊：
影响因子：
6.2
通讯作者：
Rossini, AA
中科院分区：
文献类型：
--
作者：
Markees, TG;Phillips, NE;Rossini, AA
抗CD 40配体抗体联合供体脾细胞输注可延长C57 BL/6受体上完全不匹配的BALB/c皮肤移植物的存活时间，约20%的移植物存活>100天。在体外同种异体反应性治疗的动物在移植后立即减少，但在第100天增加，尽管存在一个成功的同种异体移植。通过过继转移在体内证实了第100天同种异体反应性的存在，这表明我们的方案诱导了“分裂耐受”或“移植物适应”的状态。移植皮肤的小鼠存活时间大于或等于100天，没有发现淋巴嵌合体的迹象。尽管存在功能完整的同种异体反应性淋巴细胞，但用供体脾细胞和抗CD 40配体抗体治疗可使高抗原性供体皮肤移植物长期存活。
Combined treatment with antibody against CD40 ligand and one transfusion of donor splenocytes prolonged survival of fully mismatched BALB/c skin allografts on C57BL/6 recipients, with similar to 20% of grafts surviving >100 days. In vitro alloresponsiveness in treated animals was reduced in the immediate posttransplantation period, but by day 100 was increased despite the presence of a successful allograft. The presence of alloreactivity on day 100 was confirmed in vivo by adoptive transfer, which suggests that our protocol had induced either a state of ''split tolerance'' or ''graft accommodation.'' Mice with skin grafts that had survived for greater than or equal to 100 days revealed no evidence of lymphoid chimerism. Treatment with donor splenocytes and antibody against CD40 ligand permits longterm survival of highly antigenic donor skin allografts despite the presence of functionally intact alloreactive lymphocytes.