Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy
Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy
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DOI：
10.1063/1.1777220
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发表时间：
2004-09-01
影响因子：
4.4
通讯作者：
Zhang, XD
中科院分区：
文献类型：
--
作者：
Liu, YY;Wang, PY;Zhang, XD
分子梳是一种强大而简单的方法，用于将DNA分子排列到表面上。使用这种技术与荧光显微镜相结合，我们观察到，X-DNA分子的长度延长到约1.6倍，其轮廓长度（未延伸的长度，16.2 μ m）通过梳理方法在疏水性聚甲基丙烯酸甲酯涂层表面。研究了钠、镁离子和DNA溶液pH值的影响。有趣的是，我们观察到力诱导的单个DNA分子的熔化。(C)2004年，美国物理学会。
Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, we observed that the length of X-DNA molecules was extended to about 1.6 times their contour length (unextended length, 16.2 mum) by the combing method on hydrophobic polymethylmetacrylate coated surfaces. The effects of sodium and magnesium ions and pH of the DNA solution were investigated. Interestingly, we observed force-induced melting of single DNA molecules. (C) 2004 American Institute of Physics.