Glucocorticoid receptors in the basolateral nucleus of amygdala are required for postreactivation reconsolidation of auditory fear memory
Glucocorticoid receptors in the basolateral nucleus of amygdala are required for postreactivation reconsolidation of auditory fear memory
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DOI：
10.1111/j.1460-9568.2007.05621.x
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发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Li, Bao-Ming
中科院分区：
文献类型：
--
作者：
Jin, Xin-Chun;Lu, Yu-Fei;Li, Bao-Ming
众所周知，最初的巩固需要从头基因转录和蛋白质合成，以使记忆变得稳定。巩固的记忆再次变得不稳定，在检索时暂时对中断敏感，需要重新巩固过程才能成为永久性的。尽管杏仁核基底外侧核（BLA）中的糖皮质激素受体（GR）是恐惧记忆巩固所必需的，但关于其在恐惧记忆再巩固中的作用知之甚少。在本研究中，我们首先研究了GR拮抗剂对听觉恐惧记忆（AFM）后处理巩固的影响。GR拮抗剂RU486 0 h后处理的BLA内输注损害了长期AFM，使短期AFM完好无损。如果在预处理后6 h进行输注，则RU486没有影响。然后，我们研究了RU486治疗对AFM术后再巩固的影响。当RU486被注入BLA 0小时后，AFM的恢复（再激活）发生严重的健忘症，无论是否进行检索1天或10天后处理。如果省略记忆提取或在治疗后6小时给药，RU486不会产生健忘症。治疗RU486 0小时postretrieval没有赤字postretrieval的短期记忆，但受损postretrieval的长期记忆，和健忘症表现出没有自发恢复后6天检索。目前的结果提供了强有力的证据表明，糖皮质激素受体在BLA所需的再巩固，以及巩固AFM。
It is well known that initial consolidation requires de novo gene transcription and protein synthesis in order for memory to become stable. The consolidated memory again becomes labile and temporarily sensitive to disruption when retrieved, requiring a reconsolidation process to become permanent. Although it is well established that glucocorticoid receptors (GR) in the basolateral nucleus of amygdala (BLA) are required for consolidation of fear memory, little is known about their role in reconsolidation of fear memory. In the present study, we first examined the effect of a GR antagonist on postconditioning consolidation of auditory fear memory (AFM). Intra-BLA infusion of the GR antagonist RU486 0 h postconditioning impaired long-term AFM, leaving short-term AFM intact. RU486 had no effect if infusion was performed 6 h postconditioning. We then investigated the effect of the RU486 treatment on postretrieval reconsolidation of AFM. Severe amnesia took place when RU486 was infused into the BLA 0 h postretrieval (reactivation) of AFM, regardless of whether the retrieval was performed 1 day or 10 days postconditioning. RU486 produced no amnesia if the memory retrieval was omitted or if the drug was administered 6 h postretrieval. Treatment with RU486 0 h postretrieval produced no deficit in postretrieval short-term memory but impaired postretrieval long-term memory, and the amnesia exhibited no spontaneous recovery 6 days after retrieval. The present results provide strong evidence that glucocorticoid receptors in the BLA are required for reconsolidation as well as consolidation of AFM.