RESPONSE OF MEAT-TYPE CHICKENS TO INFECTION WITH RAV-1 AVIAN-LEUKOSIS VIRUS
RESPONSE OF MEAT-TYPE CHICKENS TO INFECTION WITH RAV-1 AVIAN-LEUKOSIS VIRUS
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DOI：
10.1080/03079459108418803
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发表时间：
1991-01-01
期刊：
影响因子：
2.8
通讯作者：
CHAMBERS, JR
中科院分区：
文献类型：
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作者：
GILKA, F;SPENCER, JL;CHAMBERS, JR
将RAV-1株禽白血病病毒在1日龄接种肉用鸡和白来航鸡，根据临床症状、血液学和尸检结果评估感染的严重程度。对选定的禽类进行了以下检查：慢性单核心肌炎的组织切片，心肌、脾、法氏囊和肾脏的群特异性病毒抗原的免疫组织化学染色切片，以及这些组织的超薄切片的病毒颗粒的电子显微镜观察。实验终止时间为115-122天。在接种的52只白来航鸡中，约有三分之一在3-4周龄时出现贫血，而177只接种了肉鸡的禽类或未接种这两种类型的禽类没有贫血的证据。血象证实了这些观察结果。发现的第一个肿瘤是骨髓瘤，在65天龄时出现在一只肉鸡身上。在接种组活到65天的151只肉鸡中，8.5%发生了肾母细胞瘤，但没有发生淋巴样白血病病例。在发生肾母细胞瘤的鸡中，心肌炎和病毒在心肌中的复制通常比没有这种病变的鸡更广泛。26~122日龄未接种对照鸡17只，心肌内未见病毒颗粒或病变。
Meat-type and White Leghorn chickens were inoculated with the RAV-1 strain of avian leukosis virus at 1 day of age and the severity of infection was assessed by clinical illness, haematology and post-mortem findings. The following were examined from selected birds: histological section for chronic mononuclear myocarditis, immunohistochemically-stained sections of myocardium, spleen, bursa of Fabricius and kidney for group-specific viral antigen, and ultrathin sections of these tissues for virus particles by electron microscopy. The experiment was terminated at 115-122 days. Approximately one-third of the 52 inoculated White Leghorns appeared anaemic at 3-4 weeks of age whereas there was no evidence of anaemia in 177 inoculated meat-type birds or in uninoculated birds of either type. Haemograms confirmed these observations.The first tumour found was a myelocytoma and it was in a meat-type bird at 65 days. Of the 151 meat-type birds of the inoculated group alive at 65 days, 8.5% developed nephroblastomas, but there were no cases of lymphoid leukosis. Myocarditis and virus replication in myocardium were usually more extensive in chickens that developed nephroblastomas than in those without such lesions. In 17 uninoculated control chickens examined between 26 and 122 days of age there were no virus particles or lesions in myocardium.