Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia.
Overexpression of a Rosa rugosa Thunb. NUDX gene enhances biosynthesis of scent volatiles in petunia.
复制标题
玫瑰的过度表达。
作者：
Sheng L;Zang S;Wang J;Wei T;Xu Y;Feng L
玫瑰是我国重要的天然香料植物。玫瑰精油被誉为“液体黄金”，具有很高的经济价值和保健价值。单萜类化合物是香茶菜的主要芳香成分。玫瑰花和精油。本研究从中国传统的红曲霉中克隆了水解酶基因家族RrNUDX 1的一个成员。唐红。结合RrNUDX 1基因表达量与花器官不同发育阶段和不同部位香气成分的分析，发现主要香气成分含量与基因表达模式一致。通过农杆菌介导的遗传转化系统获得RrNUDX 1过表达的矮牵牛。转基因矮牵牛叶片变宽，生长势强，香味浓。气相色谱-质谱联用分析表明，转基因矮牵牛中苯甲酸甲酯等主要香气成分的含量显著增加。这些结果表明，RrNUDX 1基因在增强矮牵牛花香味方面发挥了重要作用，为RrNUDX 1基因在矮牵牛中的同源异型转化奠定了基础。rugosa栽培新品种R.玫瑰品种精油产量高、质量好。
Rosa rugosa is an important natural perfume plant in China. Rose essential oil is known as ‘liquid gold’ and has high economic and health values. Monoterpenes are the main fragrant components of R. rugosa flower and essential oil. In this study, a member of the hydrolase gene family RrNUDX1 was cloned from Chinese traditional R. rugosa ‘Tang Hong’. Combined analysis of RrNUDX1 gene expression and the aroma components in different development stages and different parts of flower organ, we found that the main aroma component content was consistent with the gene expression pattern. The RrNUDX1 overexpressed Petunia hybrida was acquired via Agrobacterium-mediated genetic transformation systems. The blades of the transgenic petunias became wider and its growth vigor became strong with stronger fragrance. Gas chromatography with mass spectrometry analysis showed that the contents of the main aroma components of the transgenic petunias including methyl benzoate significantly increased. These findings indicate that the RrNUDX1 gene plays a role in enhancing the fragrance of petunia flowers, and they could lay an important foundation for the homeotic transformation of RrNUDX1 in R. rugosa for cultivating new R. rugosa varieties of high-yield and -quality essential oil.
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影响因子：
4.8
作者：
Takagi, Yasumitsu;Setoyama, Daiki;Sekiguchi, Mutsuo
通讯作者：
Sekiguchi, Mutsuo
DOI：
10.1007/978-0-387-85498-4_19
发表时间：
2009-01-01
期刊：
PLANT-DERIVED NATURAL PRODUCTS: SYNTHESIS, FUNCTION, AND APPLICATION
影响因子：
--
作者：
Negre-Zakharov, Florence;Long, Michael C.;Dudareva, Natalia
通讯作者：
Dudareva, Natalia
影响因子：
7.2
作者：
Lücker, J;Bouwmeester, HJ;Verhoeven, HA
通讯作者：
Verhoeven, HA
影响因子：
5.2
作者：
Kumar, Krishna;Kumar, Sarma Rajeev;Nagegowda, Dinesh A.
通讯作者：
Nagegowda, Dinesh A.
影响因子：
7.4
作者：
Sapir-Mir, Maya;Mett, Anahit;Eyal, Yoram
通讯作者：
Eyal, Yoram