Resistance to cypermethrin in melon thrips, Thrips palmi (Thysanoptera: Thripidae), is conferred by reduced sensitivity of the sodium channel and CYP450-mediated detoxification
Resistance to cypermethrin in melon thrips, Thrips palmi (Thysanoptera: Thripidae), is conferred by reduced sensitivity of the sodium channel and CYP450-mediated detoxification
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DOI：
10.1007/s13355-012-0141-7
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发表时间：
2012-11-01
影响因子：
1.3
通讯作者：
Sonoda, Shoji
中科院分区：
文献类型：
--
作者：
Bao, Wen Xue;Sonoda, Shoji
瓜蓟马（Thrips帕尔米Karny）是日本西部蔬菜上最具破坏性的害虫之一，因为其产生抗药性。为探讨该菌对氯氰菊酯的抗性机制，测定了两株（KC株和OK株）的钠通道基因部分核苷酸序列，并比较了推导的氨基酸序列。帕尔米。KC和OK菌株的LC 50值分别为3922.9和72.1 ppm。因此，LC 50值高于农业推荐浓度（60 ppm）。两种菌株在氨基酸位置929处具有抗性氨基酸Ile。胡椒基丁醚导致KC和OK菌株的耐药率分别降低14.1倍和3.0倍。这些结果表明，这两种菌株的基础和差分电阻水平分别由钠通道和细胞色素P450介导的解毒的敏感性降低。
Melon thrips, Thrips palmi Karny, is among the most destructive pests of vegetables in western Japan because of its development of insecticide resistance. To examine its resistance mechanisms against cypermethrin, a pyrethroid, we determined partial nucleotide sequences of the sodium channel gene and compared the deduced amino acid sequences of two strains (the KC and OK strains) of T. palmi. The KC and OK strains showed LC50 values of 3922.9 and 72.1 ppm, respectively. The LC50 values were thus above the agriculturally recommended concentration (60 ppm). Both strains had a resistant amino acid, Ile, at amino acid position 929. The synergist piperonyl butoxide caused 14.1- and 3.0-fold decreases in the resistance ratios of the KC and OK strains, respectively. These results suggest that the basal and differential resistance levels of both strains are conferred by reduced sensitivity of the sodium channel and cytochrome P450-mediated detoxification, respectively.