Microbial complexes in supragingival plaque
Microbial complexes in supragingival plaque
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DOI：
10.1111/j.1399-302x.2007.00411.x
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发表时间：
2008-06-01
影响因子：
--
通讯作者：
Song, X.
中科院分区：
文献类型：
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作者：
Haffajee, A. D.;Socransky, S. S.;Song, X.
背景/目的：研究龈上生物膜样品中的微生物群落。方法：在基线时从187名受试者（n = 4745）中采集龈上菌斑样本。55名受试者在专业牙齿清洁后1-7天提供龈上菌斑样本（n = 1456）；93名受试者在治疗后3至24个月期间提供8044份样本。采用棋盘格DNA-DNA杂交技术对所有样品进行了40种细菌的含量分析。利用聚类分析和群落排序技术分别对三组进行了物种间的微生物关联分析。结果：基线样品形成6个配合物。治疗后3-24个月的样品也形成了类似的复合物。然而，在斑块重新形成的7天内，在样品中观察到明显的微生物群落变化。与牙周病临床参数相关的复合物。结论：龈上菌斑中存在与龈下菌斑相似的特异性微生物复合物，但存在细微差异。本文还描述了以前未聚类的类群与复合体的关系。
Background/aims: To examine microbial communities in supragingival biofilm samples.Methods: Supragingival plaque samples were taken from 187 subjects at baseline (n = 4745). Fifty-five subjects provided supragingival plaque samples at 1-7 days after professional tooth cleaning (n = 1456); 93 subjects provided 8044 samples between 3 and 24 months post-therapy. All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Microbial associations among species were sought using cluster analysis and community ordination techniques for the three groups separately.Results: Six complexes were formed for the baseline samples. Similar complexes were formed for the samples taken 3-24 months post-therapy. However, distinct changes were observed in microbial communities in samples taken during the 7 days of plaque redevelopment. The complexes related to clinical parameters of periodontal disease.Conclusion: There were specific microbial complexes in supragingival plaque that were similar to those found in subgingival plaque samples with a few minor differences. The relation of previously unclustered taxa to the complexes was also described.