Characterisation and validation of Mel38; A multi-tissue microRNA signature of cutaneous melanoma
Characterisation and validation of Mel38; A multi-tissue microRNA signature of cutaneous melanoma
复制标题
Mel38 的表征和验证；
DOI：
10.1371/journal.pone.0211504
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
S. Fereday
中科院分区：
文献类型：
--
作者：
Ryan Van Laar;Mitchel Lincoln;S. Fereday
背景：黑素细胞肿瘤的组织病理学检查具有挑战性和主观性，目前没有特定的循环或组织生物标志物。最近，黑色素瘤循环38-microRNA谱（Mel38）被描述。本研究检测了Mel38在实体组织中的表达及其对下游mRNA调控的影响。方法将Mel38应用于代表良性痣、原发性和转移性黑色素瘤的存档、临床注释的实体组织基因组数据集。对与疾病状态、患者预后和分子途径相关的特征进行统计分析。结果Mel38能够根据疾病状态和黑色素瘤特异性生存差异对实体组织活检的基因组数据进行分层。实验证实Mel38的信使rna靶点也表现出预后表达模式，并代表黑色素瘤发生和进展的关键分子途径和事件。结论Mel38 microRNA谱可能在实体组织中具有诊断和预后的作用，并且是黑色素瘤的一个强大的循环生物标志物。
Background Histopathologic examination of melanocytic neoplasms can be challenging and subjective, with no specific circulating or tissue-based biomarkers currently available. Recently, a circulating 38-microRNA profile of melanoma (Mel38) was described. In this study, Mel38 expression and its impact on downstream mRNA regulation in solid tissue is examined. Methods Mel38 was applied to archival, clinically-annotated, solid-tissue genomic datasets representing benign naevi, primary and metastatic melanoma. Statistical analysis of the signature in relation to disease status, patient outcome and molecular pathways was performed. Results Mel38 is able to stratify genomic data from solid tissue biopsies on the basis of disease status and differences in melanoma-specific survival. Experimentally-verified messenger-RNA targets of Mel38 also exhibit prognostic expression patterns and represent key molecular pathways and events in melanoma development and progression. Conclusion The Mel38 microRNA profile may have diagnostic and prognostic utility in solid tissue as well as being a robust circulating biomarker of melanoma.