Very long-term stability of lipid biomarkers in human serum
Very long-term stability of lipid biomarkers in human serum
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DOI：
10.1016/j.ab.2020.113695
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发表时间：
2020-05-15
影响因子：
2.9
通讯作者：
Jansen, Eugene H. J. M.
中科院分区：
文献类型：
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作者：
Muzakova, Vladimira;Beekhof, Piet K.;Jansen, Eugene H. J. M.
生物标志物的稳定性评价对流行病学研究具有重要意义。在本研究中，在-80 ℃下储存后，在时间点0、1、8和13年，对16份人血清样本中的5个脂质参数（总胆固醇、甘油三酯、HDL-和LDL-胆固醇和游离脂肪酸）的稳定性进行了检测。除胆固醇外，大多数脂质生物标志物在储存条件下稳定。各时间点样本间的相关性非常好，因此，只要样本在-80 ℃下储存，长期储存的人血清样本就可以进行脂质生物标志物测定。
The assessment of the stability of biomarkers is very important for epidemiological studies. In this study, the stability of five lipid parameters (total cholesterol, triglycerides, HDL- and LDL-cholesterol and free fatty acids) has been tested in 16 human serum samples after storage at -80 degrees C up at time points 0, 1, 8 and 13 y.The majority of the lipid biomarkers were stable during storage conditions, except for cholesterol. The correlations between the samples were very good at time points.Therefore, long-term storage of human serum samples allows lipid biomarker determination, provided that the samples are stored at -80 degrees C.