Feeding state-dependent regulation of developmental plasticity via CaMKI and neuroendocrine signaling
Feeding state-dependent regulation of developmental plasticity via CaMKI and neuroendocrine signaling
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DOI：
10.7554/elife.10110
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发表时间：
2015-09-03
期刊：
影响因子：
7.7
通讯作者：
Sengupta, Piali
中科院分区：
文献类型：
--
作者：
Neal, Scott J.;Takeishi, Asuka;Sengupta, Piali
有关营养物质可用性的信息是通过很大程度上未知的机制来评估的，以驱动发育决策，包括选择秀丽隐杆线虫幼虫进入生殖周期或多尔阶段。在这项研究中，我们表明 CMK-1 CaMKI 将 dauer 决策调节为进食状态的函数。 CMK-1 在 ASI 感觉神经元中以细胞自主方式起作用，在 AWC 感觉神经元中以非细胞自主方式起作用，分别调节促进生长的 daf-7 TGF-β 和 daf-28 胰岛素样肽 (ILP) 基因的表达。在 AWC 中，进食状态调节 CMK-1 的动态亚细胞定位以及抗 dauer ILP 基因的 CMK-1 依赖性表达。来自 AWC 的抗 dauer ILP 信号和促 dauer 信号之间的食物调节平衡可调节神经内分泌信号传导和 dauer 进入； cmk-1 突变体中这种平衡的破坏会导致在喂养良好的条件下形成不适当的 dauer 形成。这些结果确定了营养信息整合到小神经元网络中以调节神经内分泌信号和发育可塑性的机制。
Information about nutrient availability is assessed via largely unknown mechanisms to drive developmental decisions, including the choice of Caenorhabditis elegans larvae to enter into the reproductive cycle or the dauer stage. In this study, we show that CMK-1 CaMKI regulates the dauer decision as a function of feeding state. CMK-1 acts cell-autonomously in the ASI, and non cell-autonomously in the AWC, sensory neurons to regulate expression of the growth promoting daf-7 TGF-beta and daf-28 insulin-like peptide (ILP) genes, respectively. Feeding state regulates dynamic subcellular localization of CMK-1, and CMK-1-dependent expression of anti-dauer ILP genes, in AWC. A food-regulated balance between anti-dauer ILP signals from AWC and pro-dauer signals regulates neuroendocrine signaling and dauer entry; disruption of this balance in cmk-1 mutants drives inappropriate dauer formation under well-fed conditions. These results identify mechanisms by which nutrient information is integrated in a small neuronal network to modulate neuroendocrine signaling and developmental plasticity.