Migratory responses of murine hepatic myeloid, lymphoid-related, and plasmacytoid dendritic cells to CC chemokines
Migratory responses of murine hepatic myeloid, lymphoid-related, and plasmacytoid dendritic cells to CC chemokines
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DOI：
10.1097/01.tp.0000130450.61215.3b
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发表时间：
2004-09-15
期刊：
影响因子：
6.2
通讯作者：
Thomson, AW
中科院分区：
文献类型：
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作者：
Abe, M;Zahorchak, AE;Thomson, AW
树突状细胞（DC）的运输受趋化因子受体表达和对趋化因子的反应性调节。作者比较了小鼠肝髓样、“淋巴样”和浆细胞样DC亚群的CC趋化因子受体（CCR）表达及其对CC趋化因子的反应性。RNA酶保护试验检测肝DC亚群CCR mRNA表达。在体外迁移的DC在重组鼠CC趋化因子的反应，使用Transwell室进行了测定。未成熟的肝DC没有响应任何CC趋化因子测试，尽管表达的mRNA编码适当的受体，其配体。每个肝DC亚群的CCR7表达在成熟时强烈增强。肝浆细胞样DC的迁移能力与肝髓样和淋巴样DC相似。这些发现表明，靶向CCR7及其配体可能是一种潜在的方法，用于操纵肝移植后肝DC向次级淋巴组织的运输。
Dendritic cell (DC) trafficking is regulated by chemokine receptor expression and responsiveness to chemokines. The authors compared CC chemokine receptor (CCR) expression by mouse liver myeloid, "lymphoid-related," and plasmacytoid DC subsets and their responsiveness to CC chemokines. CCR mRNA expression by liver DC subsets was evaluated by RNase protection assay. In vitro migration of the DC in response to recombinant murine CC chemokines was assayed using Transwell chambers. Immature liver DC did not respond to any CC chemokines tested, despite expression of mRNA encoding appropriate receptors for their ligands. CCR7 expression by each liver DC subset was strongly enhanced in response to maturation. The migratory capacity of liver plasmacytoid DC was similar to that of liver myeloid and lymphoid-related DC. These findings suggest that targeting of CCR7 and its ligands may be a potential approach for manipulation of liver DC trafficking to secondary lymphoid tissue after liver transplantation.