Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.
Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.
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DOI：
10.3390/ijms21217981
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发表时间：
2020-10-28
影响因子：
5.6
通讯作者：
Patarroyo MA
中科院分区：
文献类型：
--
作者：
Avendaño C;Patarroyo MA
世界卫生组织（WHO）将20种疾病列为被忽视的热带病（NTD），其中12种是寄生虫病：恰加斯病、囊虫病/带绦虫病、包虫病、食源性吸虫病、非洲人类锥虫病（昏睡病）、利什曼病、淋巴丝虫病、盘尾丝虫病（河盲症）、血吸虫病、土传蠕虫病（蛔虫、钩虫、鞭虫）、麦地那龙线虫和疥疮。此类疾病影响着发展中国家的数百万人，这些国家控制这些疾病的主要问题之一是基于诊断的问题，因为受影响最严重的地区通常远离具有合适基础设施和/或配备先进设备的实验室。在过去的二十年中，在标准化和引入能够在偏远地区进行诊断的技术方面取得了进展，即，环介导等温扩增（LAMP）技术。该技术的优点包括能够使用简单的设备进行，直接在现场进行诊断，每次测试的成本低，以及该技术的高度特异性。因此，使用这种技术可以促进被忽视的寄生虫感染（NPI）的控制和根除计划。这篇综述描述了迄今为止关于LAMP测试所取得的进展，因为人们发现，即使已经进行了几项关于大多数NPI的研究，其他人的信息是稀缺的。
The World Health Organisation (WHO) has placed twenty diseases into a group known as neglected tropical diseases (NTDs), twelve of them being parasitic diseases: Chagas’ disease, cysticercosis/taeniasis, echinococcosis, food-borne trematodiasis, human African trypanosomiasis (sleeping sickness), leishmaniasis, lymphatic filariasis, onchocerciasis (river blindness), schistosomiasis, soil-transmitted helminthiasis (ascariasis, hookworm, trichuriasis), guinea-worm and scabies. Such diseases affect millions of people in developing countries where one of the main problems concerning the control of these diseases is diagnosis-based due to the most affected areas usually being far from laboratories having suitable infrastructure and/or being equipped with sophisticated equipment. Advances have been made during the last two decades regarding standardising and introducing techniques enabling diagnoses to be made in remote places, i.e., the loop-mediated isothermal amplification (LAMP) technique. This technique’s advantages include being able to perform it using simple equipment, diagnosis made directly in the field, low cost of each test and the technique’s high specificity. Using this technique could thus contribute toward neglected parasite infection (NPI) control and eradication programmes. This review describes the advances made to date regarding LAMP tests, as it has been found that even though several studies have been conducted concerning most NPI, information is scarce for others.
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影响因子：
2.7
作者：
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