Single metallic nanoparticle imaging for protein detection in cells
Single metallic nanoparticle imaging for protein detection in cells
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DOI：
10.1073/pnas.1534635100
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发表时间：
2003-09-30
影响因子：
11.1
通讯作者：
Lounis, B
中科院分区：
文献类型：
--
作者：
Cognet, L;Tardin, C;Lounis, B
我们使用基于光热干涉对比的全光学方法对细胞中用 10 nm 金纳米颗粒标记的膜蛋白进行了可视化。该方法的高灵敏度和信号的稳定性允许对单个纳米粒子进行 3D 成像，而不会出现荧光标记固有的光漂白和闪烁的缺点。推导了一个简单的分析模型来解释信号幅度和空间分辨率的测量。光热干涉对比方法提供了一种有效、可重复且有前景的方法，通过光学手段可视化细胞中的少量蛋白质。
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.