Use of proteasome inhibitors.
Use of proteasome inhibitors.
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DOI：
10.1002/0471142735.im0910s109
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发表时间：
2015-04-01
影响因子：
--
通讯作者：
Kisselev, Alexei F
中科院分区：
文献类型：
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作者：
Downey, Sondra L;Florea, Bogdan I;Kisselev, Alexei F
蛋白酶体抑制剂是免疫学和细胞生物学中不可缺少的研究工具。随着大量蛋白酶体抑制剂的商业化，为生物实验选择合适的化合物可能是具有挑战性的，特别是对新手来说。本单元提供了研究中常用的蛋白酶体抑制剂的概述。讨论了如何为哺乳动物细胞培养实验选择合适的高度特异的抑制物，其浓度和暴露时间。此外，还讨论了可用于确认蛋白酶体抑制的检测方法。
Proteasome inhibitors are indispensable research tools in immunology and cell biology. With numerous proteasome inhibitors available commercially, choosing the appropriate compound for a biological experiment may be challenging, especially for a novice. This unit provides an overview of the proteasome inhibitors commonly used in research. It discusses how to select an appropriate highly specific inhibitor, its concentration, and length of exposure for mammalian cell culture experiments. In addition, assays that can be used to confirm proteasome inhibition are discussed.