INDIRECT ULTRAVIOLET-REACTIVATION OF PHAGE LAMBDA
INDIRECT ULTRAVIOLET-REACTIVATION OF PHAGE LAMBDA
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DOI：
10.1073/pnas.71.1.144
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
RADMAN, M
中科院分区：
文献类型：
--
作者：
GEORGE, J;DEVORET, R;RADMAN, M
当F受体大肠埃希菌从紫外线照射的供体获得hfr或F-lac+dna时，其促进紫外线损伤噬菌体λ的DNA修复和突变的能力大大增强。我们称这种现象为间接紫外线再激活，因为它的特征基本上与紫外线再激活相同；这种修复过程发生在嘧啶二聚体切除缺陷菌株中，并产生恢复噬菌体的明显斑块突变。此外，这一过程类似于间接紫外线诱导噬菌体λ，因为它是通过接合促进的。然而，与间接诱导相反，它是由HFR供体产生的，发生在限制紫外线损伤的供体DNA传入的受体中。间接紫外线再激活的发生为HFR供体DNA的存在提供了证据。一种可诱导的、容易出错的DNA修复机制。
When an F-recipientEscherichia coliK12 bacterium receives Hfr or F-lac+DNA from an ultraviolet-irradiated donor, its capacity to promote DNA repair and mutagenesis of ultraviolet-damaged phage λ is substantially increased.We call this phenomenon indirect ultraviolet-reactivation, since its features are essentially the same as those of ultraviolet-reactivation; this repair process occurs in pyrimidine dimer excision-deficient strains and produces clear plaque mutations of the restored phage. Moreover, this process is similar to indirect ultraviolet-induction of prophage λ, since it is promoted by conjugation. However, contrarily to indirect induction, it is produced by Hfr donors and occurs in recipients restricting the incoming ultraviolet-damaged donor DNA.The occurrence of indirect ultraviolet-reactivation provides evidence for the existence inE. coliof an inducible error-prone mechanism for the repair of DNA.