hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3′NTR of the HCV RNA genome
hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3′NTR of the HCV RNA genome
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DOI：
10.1093/nar/27.6.1457
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发表时间：
1999-03-15
影响因子：
14.9
通讯作者：
Del Vecchio, AM
中科院分区：
文献类型：
--
作者：
Gontarek, RR;Gutshall, LL;Del Vecchio, AM
与黄病毒科的其他成员一样，丙型肝炎病毒（HCV）的3'非翻译区（NTR）被认为通过与病毒复制酶复合物的组分相互作用而在病毒RNA复制的起始和调节中起作用。为了研究宿主成分可能也参与这一过程的可能性，我们使用UV交联测定来确定是否有任何细胞蛋白质可以特异性结合3 'NTR RNA。我们证明了两种宿主蛋白与HCV 3 'NTR内广泛的富含嘧啶的区域的特异性相互作用。一种宿主蛋白以分子量为57 kDa的双联体形式迁移，并与多聚嘧啶片段结合蛋白（PTB）特异性抗血清具有免疫反应性，另一种蛋白（35 kDa）被特异性针对异质核核糖核蛋白C（hnRNP C）的单克隆抗体识别，这些结果表明PTB和hnRNP C对大的嘧啶富集区的识别可能在HCV RNA复制的启动和/或调节中起作用。
Like other members of the Flaviviridae family, the 3' non-translated region (NTR) of the hepatitis C virus (HCV) is believed to function in the initiation and regulation of viral RNA replication by interacting with components of the viral replicase complex. To investigate the possibility that host components may-also participate in this process, we used UV cross-linking assays to determine if any cellular proteins could bind specifically to the 3'NTR RNA. We demonstrate the specific interaction of two host proteins with the extensive pyrimidine-rich region within the HCV 3'NTR. One host protein migrates as a doublet with a molecular weight of 57 kDa and is immunoreactive with antisera specific for polypyrimidine tract-binding protein-(PTB), and the other protein (35 kDa) is recognized by a monoclonal antibody specific for heterogeneous nuclear ribonucleoprotein C (hnRNP C), These results suggest that recognition of the large pyrimidine-rich region by PTB and hnRNP C may play a role in the initiation and/or regulation of HCV RNA replication.