A double-targeted magnetic nanocarrier with potential application in hydrophobic drug delivery
A double-targeted magnetic nanocarrier with potential application in hydrophobic drug delivery
复制标题
一种双靶向磁性纳米载体在疏水性药物输送中的潜在应用
DOI：
10.1016/j.colsurfb.2011.10.036
复制
发表时间：
2012-03-01
影响因子：
5.8
通讯作者：
Zhang, Xuezhong
中科院分区：
文献类型：
--
作者：
Ding, Guobin;Guo, Yi;Zhang, Xuezhong
基于磁性纳米载体的双靶向给药系统在疏水性药物释放方面具有潜在的应用前景。它由磁铁矿（Fe 3 O 4）纳米粒子封装在两亲性共聚物MPEG-PLGA [甲氧基聚（乙二醇）-聚（D，L-丙交酯-共-乙交酯）]的自组装胶束，并使用溶剂蒸发技术制备。磁性纳米载体具有非常稳定的核壳结构，并且是超顺磁性的。用MTT法对HeLa、MCF-7和HT 1080三种细胞系进行细胞毒性评价;孵育24 h后，浓度高达400 μ g/mL时，对任何受试细胞系均无细胞毒性。通过普鲁士蓝染色和将荧光探针（疏水量子点）封装到纳米载体中后通过荧光显微镜研究其细胞摄取。最后，通过体外实验证实了磁性纳米载体的磁靶向性。总体而言，所获得的结果证明了双靶向纳米载体用于疏水性药物的细胞内递送的潜力。(C)2011爱思唯尔有限公司版权所有。
A double-targeted magnetic nanocarrier based with potential applications in the delivery of hydrophobic drugs has been developed. It consists of magnetite (Fe3O4) nanoparticles encapsulated in self-assembled micelles of the amphiphilic copolymer MPEG-PLGA [methoxy poly (ethylene glycol)-poly (D,L-lactide-co-glycolide)], and was fabricated using the solvent-evaporation technique. The magnetic nanocarrier has a very stable core-shell structure and is superparamagnetic. Its cytotoxicity was evaluated using the MTT assay with three cell lines-HeLa, MCF-7, and HT1080; it exhibited no cytotoxicity against any tested line at concentrations of up to 400 mu g/mL after incubation for 24 h. Its cellular uptake was studied by Prussian blue staining and by fluorescence microscopy after encapsulating a fluorescent probe (hydrophobic quantum dots) into the nanocarrier. Finally, the magnetic targeting property of the magnetic nanocarrier was confirmed by an in vitro test. Overall, the results obtained demonstrate the potential of the double-targeted nanocarrier for the intracellular delivery of hydrophobic drugs. (C) 2011 Elsevier B.V. All rights reserved.