A fluorescently‐labelled r‐RNA targeted oligonucleotide probe for the in situ detection of G‐bacteria of the genus Amaricoccus in activated sludge
A fluorescently‐labelled r‐RNA targeted oligonucleotide probe for the in situ detection of G‐bacteria of the genus Amaricoccus in activated sludge
复制标题
荧光标记的 r-RNA 靶向寡核苷酸探针，用于原位检测活性污泥中 Amaricoccus 属的 G 细菌
DOI：
10.1046/j.1365-2672.2000.01022.x
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发表时间：
2000
影响因子：
4
通讯作者：
J. Wanner
中科院分区：
文献类型：
--
作者：
A. Maszenan;R. Seviour;B. Patel;J. Wanner
描述了一种对Amaricoccus属成员具有特异性的荧光标记的r-RNA靶向寡核苷酸探针，其中包括活性污泥系统中观察到的一组革兰氏阴性G-细菌。这些生物体，先前在四分体中的球菌的独特形态上被“鉴定”，通过与聚磷酸盐积累细菌竞争，与生物营养物去除（EBNR）工厂的不良性能相关。详细介绍了用于探测活性污泥的样品制备方法，并使用该探针对46种植物进行了初步调查，结果表明，属于Amaricoccus属的G-细菌不仅在EBNR系统中大量存在，而且在常规植物中也存在。这种生物体的单细胞的存在是常见的，强调了依赖形态学和细胞排列来识别这些细菌的危险性。
A fluorescently‐labelled r‐RNAtargeted oligonucleotide probe specific for members of the genus Amaricoccus, which includes one group of the Gram‐negative G‐Bacteria seen in activated sludge systems, is described. These organisms, previously ‘identified’ on their distinctive morphology of cocci in tetrads, have been associated with poor performance of biological nutrient removal (EBNR) plants, by out‐competing the polyphosphate accumulating bacteria. Methods of sample preparation for probing activated sludge are detailed, and preliminary surveys of 46 plants, using this probe, show that G‐Bacteria belonging to the genus Amaricoccus are seen not only in large numbers in EBNR systems but also in conventional plants. The presence of single cells of this organism was common, emphasizing the dangers of relying on morphology and cell arrangement to identify these bacteria.